merck millipore,默克密理博,AB1761,Anti-Protein Gene Product 9.5 Antibody

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merck millipore,默克密理博,AB1761,Anti-Protein Gene Product 9.5 Antibody
产品名称：Anti-Protein Gene Product 9.5 Antibody
产品型号：AB1761
This Anti-Protein Gene Product 9.5 Antibody is validated for use in IH, IH(P), WB for the detection of Protein Gene Product 9.5.

merck millipore,默克密理博,AB1761,Anti-Protein Gene Product 9.5 Antibody

产品介绍

merck millipore,默克密理博,AB1761,Anti-Protein Gene Product 9.5 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB, IHC	Rb	Serum	Polyclonal Antibody

描述
产品目录编号	AB1761
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Protein Gene Product 9.5 Antibody
Alternate Names	UCH-L1 Ubiquitin C-terminal Hydrolase PGP 9.5
背景信息	Protein Gene Product 9.5 (PGP9.5) is an abundant protein in many tissues, but especially so in neurons where it has been effectively used as a phenotypic marker and is immunologically distinct from neuron specific enolase. The 27 kDa protein was first identified by high resolution two dimensional PAGE. Standard immunohistochemical techniques have demonstrated the presence of PGP9.5 in neurons and nerve fibers at all levels of the central and peripheral nervous system, in many neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. PGP9.5 is known to be a member of the ubiquitin C-terminal hydrolase family and immunohistochemical studies have shown that the protein is enriched in several ubiquinated inclusion bodies, suggesting that such structures may be metabolically dynamic regions of the cell.

产品信息
格式	Serum
控制	Brain tissue.
演示	Rabbit polyclonal serum in buffer containing 0.01 M sodium azide.

应用
应用	This Anti-Protein Gene Product 9.5 Antibody is validated for use in IH, IH(P), WB for the detection of Protein Gene Product 9.5.
主要应用	Western Blotting Immunohistochemistry
应用说明	Western Blot: 1:1,000. Recognizes a 24-27 kDa protein. Immunohistochemistry (paraffin): Citrate pH 6.0 antigen retrieval followed by incubation with the antibody at a dilution of 1:50 - 1:500. Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	Recombinant human UCH-L1.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Protein Gene Product 9.5 (PGP9.5, Ubiquitin C-terminal Hydrolase, UCH-L1) is an abundant protein in many tissues, but especially so in neurons where it has been effectively used as a phenotypic marker. PGP9.5 is known to be a member of the ubiquitin C-terminal hydrolase family and immunohistochemical studies have shown that the protein is enriched in several ubiquinated inclusion bodies, suggesting that such structures may be metabolically dynamic regions of the cell.
品种反应性	RatHumanMouse
Species Reactivity Note	Human, rat and mouse. Other species have not been tested.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_004172
Entrez基因汇总	UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).[supplied by OMIM]
基因符号	EC 6.-.-.- UCHL1 PARK5 UCH-L1 Uch-L1 PGP9.5 EC 3.4.19.12
纯化方法	Unpurified
UniProt编号	P09936
UniProt汇总	FUNCTION: SwissProt: P09936 # Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. SIZE: 223 amino acids; 24824 Da SUBUNIT: Homodimer. Interacts with SNCA (By similarity). Interacts with COPS5. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. PTM: O-glycosylated (By similarity). DISEASE: SwissProt: P09936 # Oxidation of Met-1, Met-6, Met-12, Met-124 and Met-179 to methionine sulfoxide, and oxidation of Cys-220 to cysteine sulfonic acid have been observed in brains from Alzheimer disease (AD) and Parkinson disease (PD) patients. In AD, UCHL1 was found to be associated with neurofibrillary tangles. SIMILARITY: SwissProt: P09936 ## Belongs to the peptidase C12 family. MISCELLANEOUS: In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.

产品使用声明
质量保证	Routinely evaluated by Western Blot on Mouse Brain lysates. Western Blot Analysis: 1:1000 dilution of this lot detected PROTEIN GENE PRODUCT 9.5 on 10 μg of Mouse Brain lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C in undiluted aliquots (not less than 10 μL) from date of receipt. The use of high quality “antiserum-grade” plastic or glass vials is recommended. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

储存和货运信息

存储条件

Stable for 1 year at -20°C in undiluted aliquots (not less than 10 μL) from date of receipt. The use of high quality “antiserum-grade” plastic or glass vials is recommended.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

包装信息
数量	50 µL

merck millipore,默克密理博,AB1761,Anti-Protein Gene Product 9.5 Antibody

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