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品种反应性 主要应用 宿主 格式 抗体类型 R, B, M ICC, ELISA, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB1727 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Connexin 43 Antibody 概述 Mouse Connexin 43 is a 382 amino acid gap junction protein withmouse connexin
a predicted M.W. of ~43 kDa. It is prominently expressed in heart (see reviews: Kumar & Giula 1996; White et al. 1995; Evans 1994; Beyer et al. 1990).Alternate Names - Gap Junction alpha-1 Protein (CxA-1)
产品信息 格式 Affinity Purified 演示 Affinity-purified using peptide-Sepharose column chromatography and supplied in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl containing 0.05% sodium azide. 应用 应用 This Anti-Connexin 43 Antibody is validated for use in ELISA, IP, WB, IC for the detection of Connexin 43. 主要应用 - Immunocytochemistry
- ELISA
- Western Blotting
应用说明 ELISA: 1:10,000-100,000 using 50 - 100 ng Cx43 control peptide per well.
Immunocytochemistry: not tested. It is recommended that the antibody be tried at 2-20μg/mL in formaldehyde fixed (Beyer et al. 1985; Nicholson et al. 1985; John et al. 1991; Fishman et al. 1990).
Immunoblotting: 1-10μg/mL using Chemiluminescence technique.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 KLH-conjugated synthetic peptide
corresponding to amino acids 360-382 within
the C-terminus of mouse connexin 43.宿主 Rabbit 特异性 Mouse Cx43 immunogenic peptide sequence is specific for Cx43 and no significant homology is seen with other connexins. The mouse Cx43 peptide sequence shows 100% conserved with rat and bovine, and 84% with chicken and human (16/19 aa) Cx43 (Beyer et al. 1985; Nicholson et al. 1985; John et al. 1991; Fishman et al. 1990). 品种反应性 RatBovineMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000165.3
Entrez基因汇总 This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations. 基因符号 - Connexin-43
- GJA1
- GJAL
- Cx43
- DFNB38
- SDTY3
- CX43
- ODD
- ODDD
- ODOD
UniProt编号 - P17302
UniProt汇总 FUNCTION: SwissProt: P17302 # One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
SIZE: 382 amino acids; 43008 Da
SUBUNIT: A connexon is composed of a hexamer of connexins. Interacts with SGSM3.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
TISSUE SPECIFICITY: Expressed in the heart and fetal cochlea.
DISEASE: SwissProt: P17302 # Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type III and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances. & Defects in GJA1 may be the cause of syndactyly type III (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected. & Defects in GJA1 a the cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
SIMILARITY: SwissProt: P17302 ## Belongs to the connexin family. Alpha-type (group II) subfamily.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at 2-8°C for 1 year from date of receipt. 包装信息 数量 50 µg
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