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merck millipore,默克密理博,MAB429,Anti-Progesterone Receptor Antibody, clone 1A6
产品名称：Anti-Progesterone Receptor Antibody, clone 1A6
产品型号：MAB429
Anti-Progesterone Receptor Antibody, clone 1A6 is a high quality Mouse Monoclonal Antibody for the detection of Progesterone Receptor & has been validated in IHC, IHC(P).
merck millipore,默克密理博,MAB429,Anti-Progesterone Receptor Antibody, clone 1A6
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H IHC M Culture Supernatant Monoclonal Antibody 描述 产品目录编号 MAB429 Replaces 04-1018 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Progesterone Receptor Antibody, clone 1A6 产品信息 格式 Culture Supernatant 控制 - POSITIVE CONTROL:
Lobular and tubular breast carcinomas tend to be positive.
演示 Culture supernatant, with 15 mM sodium azide, liquid. 应用 应用 Anti-Progesterone Receptor Antibody, clone 1A6 is a high quality Mouse Monoclonal Antibody for the detection of Progesterone Receptor & has been validated in IHC, IHC(P). 主要应用 - Immunohistochemistry
应用说明 Immunohistochemistry
Frozen sections: 1:40, (fixed for 10 minutes at room temperature with Zamboni's) 60 minutes incubation at 25°C. Indirect immunoperoxidase technique.
INDIRECT IMMUNOPEROXIDASE TECHNIQUE FOR MAB429
1. Cut 7 micron sections of frozen tissue and fix immediately in Zamboni's fixative for 10 min.
2. Wash 3 x 10 min. in TBS.
3. Cover with NGS for 10 min.
4. Remove excess serum, cover with MAB429 (Diluted 1:40 with NGS) and incubate 1 hour at room temp.
5. Wash in TBS for 2 x 5 min.
6. Cover with secondary antibody and incubate for 30 min. at room temperature.
7. Wash in TBS for 2 x 5 min.
8. Develop with DAB.
9. It is suggested that a counterstain is not used.
10. Dehydrate, clear and mount.
*Note: NGS = Normal Goat Serum diluted 1 in 5 with TBS.
ZAMBONI'S FIXATIVE
1. Mix 20 G paraformaldehyde with 150 mL double-filtered, saturated aqueous picric acid.
2. Heat to 60° C in fume cupboard.
3. Add 2.52% sodium hydroxide in water, drop by drop until solution is clear.
4. Filter solution and allow to cool.
5. Make up to 1000 mL with phosphate buffer. (3.31g NaH2 PO4.H2O, 33.77g Na2HPO4.7H2O 1000 mL)
Reference: 1) Nature (1967) 216:174-175
Paraffin embedded sections: 1:40 with high temperature antigen unmasking technique. 60 minutes incubation at 25°C. Standard ABC technique.
Western blotting: 1:25-1:50
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide corresponding to a site of predicted high antigenicity on the human progesterone receptor. 克隆 1A6 宿主 Mouse 特异性 Human progesterone receptor protein.
STAINING PATTERN:
Nuclear同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000926.4
Entrez基因汇总 This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform A only, and mediate their own response genes and physiologic effects with little overlap. The location of transcription initiation for isoform B has not been clearly determined. 基因符号 - NR3C3
- PGR
- PR
UniProt编号 - P06401
UniProt汇总 FUNCTION: SwissProt: P06401 # The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
SIZE: 933 amino acids; 98981 Da
SUBUNIT: Interacts with CUEDC2, SMARD1 and with UNC45A.
SUBCELLULAR LOCATION: Nucleus.
DOMAIN: SwissProt: P06401 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.
SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 500 µL
上一件merck millipore产品：merck millipore,默克密理博,17-126,PTP Assay Kit 2
下一件merck millipore产品：merck millipore,默克密理博,04-808,Anti-dimethyl-Histone H3 (Arg2), clone 20.2 Antibody, rabbit monoclonal
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