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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB16961 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-DFF45/35 Antibody, NT 产品信息 格式 Purified 演示 Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide. 应用 应用 This Anti-DFF45/35 Antibody, N-terminus is validated for use in IP, WB for the detection of DFF45/35. 主要应用 - Western Blotting
应用说明 Western blot: 1:500-1:1000
Immunoprecipitation
Whole cell lysate from HeLa cells can be used as a positive control and a 45 kDa band should be detected.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Polyclonal antibody raised against a peptide corresponding to amino acids 2-21 at N-terminus of human DFF (Liu et al. 1997). 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis (Liu et al. 1997). Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD (Enari et al. 1998; Sakahira et al. 1998). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (Enari et al. 1998; Sakahira et al. 1998). Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which cause DNase activation and DNA degradation, is the hallmark of apoptotic cell death (Wylile et al. 1998). 品种反应性 Human 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_213566.1
- NM_004401.2
Entrez基因汇总 Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. 基因符号 - DFF1
- DFFA
- DFF45
- DFF-45
- ICAD
UniProt编号 - O00273
UniProt汇总 FUNCTION: SwissProt: O00273 # Inhibitor of the caspase-activated DNase (DFF40).
SIZE: 331 amino acids; 36522 Da
SUBUNIT: Heterodimer of DFFA and DFFB.
SUBCELLULAR LOCATION: Cytoplasm.
PTM: Caspase-3 cleaves DFF45 at 2 sites to generate an active factor. & Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: O00273 ## Contains 1 CIDE-N domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. 包装信息 数量 100 µg
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