merck millipore,默克密理博,70091,pET-40b(+) DNA

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merck millipore,默克密理博,70091,pET-40b(+) DNA - Novagen
产品名称：pET-40b(+) DNA - Novagen
产品型号：70091
Novagen's pET Dsb Fusion Systems 39b and 40b are designed for cloning and high-level expression of peptide sequences fused with the 208-aa Dsb-Tag sequence.

merck millipore,默克密理博,70091,pET-40b(+) DNA - Novagen

产品介绍

merck millipore,默克密理博,70091,pET-40b(+) DNA - Novagen

描述
概述	The pET Dsb Fusion Systems 39b and 40b are designed for cloning and high-level expression of peptide sequences fused with the 208-aa DsbA•Tag™ sequence [pET-39b(+)] or the 236-aa DsbC•Tag™ sequence [pET-40b(+)]. DsbA and DsbC are periplasmic enzymes that catalyze the formation and isomerization of disulfide bonds, respectively (Rietsch 1996, Sone 1997, Missiakas 1994, Zapun 195, Raina 1997). In contrast to the pET-32 Trx•Tag™ series, which is designed to increase solubility in the cytoplasm, the DsbA•Tag and DsbC•Tag vectors enable potential periplasmic localization and thus may enhance solubility and proper folding in this non-reducing environment (Collins-Racie 1995). The pET-40b(+) vector is designed for expression of DsbC fusion proteins. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB229). The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information. Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
产品目录编号	70091
品牌系列	Novagen®

描述

概述

The pET Dsb Fusion Systems 39b and 40b are designed for cloning and high-level expression of peptide sequences fused with the 208-aa DsbA•Tag™ sequence [pET-39b(+)] or the 236-aa DsbC•Tag™ sequence [pET-40b(+)].

DsbA and DsbC are periplasmic enzymes that catalyze the formation and isomerization of disulfide bonds, respectively (Rietsch 1996, Sone 1997, Missiakas 1994, Zapun 195, Raina 1997). In contrast to the pET-32 Trx•Tag™ series, which is designed to increase solubility in the cytoplasm, the DsbA•Tag and DsbC•Tag vectors enable potential periplasmic localization and thus may enhance solubility and proper folding in this non-reducing environment (Collins-Racie 1995).

The pET-40b(+) vector is designed for expression of DsbC fusion proteins. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB229). The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand.

The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.

Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.

产品目录编号

70091

品牌系列

Novagen®

参考
参考	Rietsch, A., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 13048. Sone, M., et al. 1997. J. Biol. Chem. 272, 10349. Missiakas, D., et al. 1994. EMBO J. 13, 2013. Zapun, A., et al. 1995. Biochemistry 34, 5075. Raina, S. and Missiakas, D. 1997. Ann. Rev. Microbiol. 51, 179. Collins-Racie, L.A., et al. 1995. Bio/Technology 13, 982.