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描述 概述 The pIEx™ vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. The pIEx-10 contains the coding sequence for the mouse IgM signal sequence to allow secretion of the expressed protein. It also encodes an N-terminal 8 aa Strep•Tag® II coding sequence followed by an enterokinase protease cleavage site and a multiple cloning site, with the option to include a C-terminal His•Tag® coding sequence with 10 histidines. Additionally, pIEx-10 is available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation. 产品目录编号 71557 品牌系列 Novagen®
储存和货运信息 货运代码 Shipped with Blue Ice or with Dry Ice
毒性 Irritant
储存 -20°C
无需冷冻 No
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