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品种反应性 主要应用 宿主 格式 抗体类型 R WB, ELISA, IHC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB3067 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Aquaporin 3 Antibody Alternate Names - GLIP
- AQP3
产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA. 应用 应用 This Anti-Aquaporin 3 Antibody is validated for use in ELISA, IH, WB for the detection of Aquaporin 3. 主要应用 - Western Blotting
- ELISA
- Immunohistochemistry
应用说明 Western Blotting: 1-10 μg/mL for affinity pure antibody using Chemiluminescence technique. An antibody made to the AQP3 peptide detected a major band at ~27 kDa and a broader band at 33-40 kDa in membranes from outer rat medulla (Ecelbarger et al. 1995), in rat kidney cortex, and 35-47 kDa in inner and outer medulla (postranslational or glycosylation) (Ecelbarger et al. 1995). A similar heterogeneity, attributed to glycosylation, has been observed for AQP2.
Immunohistochemistry: 2-10 μg/mL in paraformaldehyde fixed sections of tissues. AQP2 has been localized in basolateral region of collecting ducts (Ishibashi et al. 1994; Echevarria et al. 1994; Ma et al. 1994; Ecelbarger et al. 1995).
ELISA: 1:100K using 10-100ng/mL control peptide (AG776)/well.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 A 15 aa C-terminal peptide from rat AQP3. This peptide is predicted to be cytoplasmic. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Aquaporin 3 (AQP3/GLIP). 品种反应性 Rat Species Reactivity Note The antibody has not yet been tested for cross-reactivity to other species. The immunogen peptide has 92% similarity with mouse AQP3 and 85% with human and ovine AQP3. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004925.3
Entrez基因汇总 Aquaporin 3 is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. 基因符号 - GIL
- AQP3
- AQP-3
- Aquaporin-3
UniProt编号 - Q92482
UniProt汇总 FUNCTION: SwissProt: Q92482 # Forms a water-specific channel that provide kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. May function as a water and urea exit mechanism in antidiuresis in collecting duct cells. Also slightly permeable to urea and glycerol. May play an important role in gastrointestinal tract water transport and in glycerol metabolism (By similarity).
SIZE: 292 amino acids; 31544 Da
SUBCELLULAR LOCATION: Basolateral cell membrane; Multi-pass membrane protein. Note=In collecting ducts of kidney.
TISSUE SPECIFICITY: Widely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level.
DOMAIN:SwissProt: Q92482 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µg
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