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品种反应性 主要应用 宿主 格式 抗体类型 A WB, ELISA Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB1599 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Phosphotyrosine Antibody 产品信息 格式 Affinity Purified 控制 - POSITIVE: EGF-stimulated A431 cells.
演示 Affinity purified immunoglobulin in 0.05m Tris with 0.15M NaCl, pH=7.2, and 0.02% sodium azide. 应用 应用 Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody validated for use in ELISA & WB. 主要应用 - Western Blotting
- ELISA
应用说明 Western blot: 1:5,000 - 1:30,000 using colorimetric detection. Detection with more sensitive systems, such as chemiluminescence, may require higher antibody dilution. At a dilution of 1:1,000 the antibody was easily able to detect 10 ng (0.2 pmol) of a phosphorylated peptide using chromogenic (BCIP/NBT) development.
Slot blot: 1:10,000.
ELISA: 1:30,000 to 1;100,000 for detection of 1 μg/well of phosphotyrosine-containing substrate.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 A random amino acid polymer containing phosphotyrosine which was conjugated to Keyhole Limpet Hemocyanin. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Specific for phosphotyrosine. No reaction with phosphoserine, phosphothreonine, serine, threonine, or tyrosine as confirmed by ELISA and Western blot using phosphorylated and unphosphosphorylated proteins. 品种反应性 All 抗体类型 Polyclonal Antibody 修改 - Phosphorylation
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -20°C in undiluted aliquots. For working stocks, it is suggested to dilute the stock 20μL with a solution of 1% BSA in TBS (pH 7.4, that has been filtered through a 0.45μm membrane filter) to enhance the full recovery of the antibody from the supplied tube. Avoid repeated freeze/thaw. 包装信息 数量 100 µg
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