merck millipore,默克密理愽
  • merck millipore,默克密理博,AB1582,Anti-Choline Acetyltransferase Antibody

  • merck millipore,默克密理博,AB1582,Anti-Choline Acetyltransferase Antibody

    产品名称：Anti-Choline Acetyltransferase Antibody
    产品型号：AB1582
    This Anti-Choline Acetyltransferase Antibody is validated for use in IH, WB for the detection of Choline Acetyltransferase.

    merck millipore,默克密理博,AB1582,Anti-Choline Acetyltransferase Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB1582,Anti-Choline Acetyltransferase Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Rb, Gp, RWB, IHCShSerumPolyclonal Antibody
    描述
    产品目录编号 AB1582
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Choline Acetyltransferase Antibody
    Alternate Names
    • ChAT
    产品信息
    格式 Serum
    演示 Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any residue.
    应用
    应用 This Anti-Choline Acetyltransferase Antibody is validated for use in IH, WB for the detection of Choline Acetyltransferase.
    主要应用
    • Western Blotting
    • Immunohistochemistry
    应用说明 Immunohistochemistry: 1:1,000-1:2,000

    Immunoblotting

    Optimal working dilutions must be determined by end user.

    IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1582

    STAINING OF GUINEA PIG ILEUM

    1) Pin the ileum out flat and tight.

    2) Fix overnight at 4°C in modified Zamboni's (2% paraformaldehyde with 15% saturated picric acid in phosphate buffered saline).

    3) Clear in DMSO, 100%, 3 times for 10 minutes each, rinse in PBS.

    4) Dissect off ALL circular muscle.

    5) Preincubate with 10% normal donkey serum.

    6) Incubate in anti-ChAT (AB1582) diluted 1:1,000-1,2000 with 10% normal donkey serum over night at 4°C on a shaker.

    7) Incubate with Donkey anti-Sheep IgG-Cy3 labeled secondary antibody (Catalog Number AP184C) at a dilution of 1:200 for 2 hours.

    IMPORTANT NOTE: It is essential to remove ALL circular muscle.
    生物信息
    免疫原品种 A 22 amino acid peptide from porcine ChAT, coupled to KLH. The sequence is H-GLFSSYRLPGHTQDTLVAQKSS-NH2.
    宿主 Sheep
    特异性 Choline Acetyltransferase (ChAT). Will stain cholinergic neurons in rat, guinea-pig and rabbit central and peripheral nervous systems.
    品种反应性 RabbitGuinea PigRat
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_020986.2
    • NM_020549.3
    • NM_020984.2
    • NM_020985.2
    Entrez基因汇总 Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.
    基因符号
    • EC 2.3.1.6
    • CHAT
    • ChAT
    • CMS1A2
    • CHOACTase
    • CMS1A
    UniProt编号
    • P28329
    UniProt汇总 FUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
    SIZE: 748 amino acids; 82568 Da
    DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
    SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,AB1582,Anti-Choline Acetyltransferase Antibody

上一件merck millipore产品：merck millipore,默克密理博,ABN334,Anti-SAPAP4 Antibody
下一件merck millipore产品：merck millipore,默克密理博,238804,Cdk2 Inhibitor IV, NU6140 - CAS 444723-13-1 - Calbiochem


相关产品

merck millipore,默克密理博,23-029M,UbcH5a Protein, active, 250 µg merck millipore,默克密理博,23-029M,UbcH5a Protein, active, 250 µg

merck millipore,默克密理博,HTS082RTA,Ready-to-Assay™ 5-HT2A Serotonin Family Receptor Frozen Cells merck millipore,默克密理博,HTS082RTA,Ready-to-Assay™ 5-HT2A Serotonin Family Receptor Frozen Cells

merck millipore,默克密理博,ST1637,Anti-ITGA7 Mouse mAb (8G2) merck millipore,默克密理博,ST1637,Anti-ITGA7 Mouse mAb (8G2)

merck millipore,默克密理博,AB10537,Anti-Sox4 Antibody merck millipore,默克密理博,AB10537,Anti-Sox4 Antibody

merck millipore,默克密理博,ABN334,Anti-SAPAP4 Antibody merck millipore,默克密理博,ABN334,Anti-SAPAP4 Antibody

merck millipore,默克密理博,238804,Cdk2 Inhibitor IV, NU6140 - CAS 444723-13-1 - Calbiochem merck millipore,默克密理博,238804,Cdk2 Inhibitor IV, NU6140 - CAS 444723-13-1 - Calbiochem

merck millipore,默克密理博,AP112,Goat Anti-Human IgG Antibody merck millipore,默克密理博,AP112,Goat Anti-Human IgG Antibody

merck millipore,默克密理博,263275,Desmosine merck millipore,默克密理博,263275,Desmosine

merck millipore,默克密理博,7490-OP,OmniPur Silver Pro Stain Kit - Calbiochem merck millipore,默克密理博,7490-OP,OmniPur Silver Pro Stain Kit - Calbiochem

merck millipore,默克密理博,LSK2ABG20,Montage 抗体纯化试剂盒与 PROSEP-G 介质 merck millipore,默克密理博,LSK2ABG20,Montage 抗体纯化试剂盒与 PROSEP-G 介质


whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech


上海羽令过滤器材有限公司版权所有 Copyright ©  www.yl-guolv.com  Powered by 羽令集团—信息技术部