- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R WB, ELISA, ICC, IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB1558 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Dopamine D2 Receptor Antibody 产品信息 格式 Serum 控制 - Brain
演示 Rabbit antiSerum. Liquid containing 0.05% sodium azide. 应用 应用 Anti-Dopamine D2 Receptor Antibody is an antibody against Dopamine D2 Receptor for use in ELISA, IC, IH & WB. 主要应用 - Western Blotting
- ELISA
- Immunocytochemistry
- Immunohistochemistry
应用说明
Immunohistochemistry: 4% PFA fixed, frozen sections, 1:200, 24 hour incubation at room temperature, DAB detection. {Tillerson, JL et al. (2006) Behavioural Brain Res. 172:97-105.
Immunocytochemistry: 4% PFA fixed cells 10-15', permeabilized with 0.2-0.3% triton X-100, 1:100, o/N 4C for fluorescence {Sun, W et al (2005) Stem Cells 23(7):931-945}.
Western blot: 1:1,000-1:5,000 (Chemiluminescence technique) Higher concentration of antibody may be necessary when using low concentration samples or when using milk in antibody diluents. It may be necessary to load 100-200 μg of purified striatal or other tissue membranes to visualize the DR2 bands. The antibody has been shown to recognize both native and denatured DR2 bands.
ELISA: 1:10,000-1:50,000 [1 μg/mL D2R peptide (Cat. Number AG284)/well]
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Amino-terminal peptide sequence (aa 24-34) near or at the ligand binding domain of rat D2R (1,2).
The immunogen shows 100% homology with mouse, 90% with human and bovine and 72% with canine D2R receptors. An antibody made to the same sequence has shown reactivity with human, lobster, squid and crawfish.浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Dopamine D2 Receptor (D2R). Recognizes both native and denatured forms of D2R (110, 68 and 47 kDa bands by Western blot using rat striatal positive control (Catalog Number AG282)). AB1558 inhibits binding of [3H]YM-09152-2, a known D2R antagonist and (125)I-NAPS, a photoligand. It inhibits binding of [3H]YM-09152-2 and PPHT-fluorescein (an agonist of D2R) and reacts with 110 kDa D2R band in fibroblasts transfected with rat D2R cDNA. AG284 is available for blocking experiments; AG284 is not recommended as a positive control for western blots. 品种反应性 Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_016574.2
- NM_000795.2
Entrez基因汇总 This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing. 基因符号 - D2DR
- DRD2
- D
- D2R
纯化方法 Unpurified UniProt编号 - P14416
UniProt汇总 FUNCTION: SwissProt: P14416 # This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
SIZE: 443 amino acids; 50619 Da
SUBUNIT: Interacts with GPRASP1, neurabin-2 and CLIC6 (By similarity). Interacts with CADPS and CADPS2.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
DISEASE: SwissProt: P14416 # It has been suggested that DRD2 is involved in psychiatric disorders; especially in schizophrenia. & Defects in DRD2 are associated with myoclonus dystonia (MD) [MIM:159900]; also known as myoclonic dystonia, alcohol- responsive dystonia and dystonia-11 (DYT11). MD is a movement disorder characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable.
SIMILARITY: SwissProt: P14416 ## Belongs to the G-protein coupled receptor 1 family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 50 µL
上一件merck millipore产品：merck millipore,默克密理博,17-610,Magna ChIP™ A - Chromatin Immunoprecipitation Kit
下一件merck millipore产品：merck millipore,默克密理博,FCMAB310A4,Milli-Mark™ Mouse IgG1-k, clone MOPC-21, Alexa Fluor 488 conjugate
相关产品
merck millipore,默克密理博,ABT348,Anti-AGBL2 Antibody
merck millipore,默克密理博,MDRPNP410,MultiScreen 深孔 Solvinert，疏水性 PTFE，0.45 µm，带预过滤膜，无色，非无菌
merck millipore,默克密理博,GF141,Stem Cell Factor Protein, Recombinant mouse
merck millipore,默克密理博,05-23-0756,[Nle⁴,D-Phe⁷]-α-Melanocyte-Stimulating Hormone
merck millipore,默克密理博,17-610,Magna ChIP™ A - Chromatin Immunoprecipitation Kit
merck millipore,默克密理博,FCMAB310A4,Milli-Mark™ Mouse IgG1-k, clone MOPC-21, Alexa Fluor 488 conjugate
merck millipore,默克密理博,FCRNC25112,FlowCellect Rodent NSC Characterization Kit (Neural)
merck millipore,默克密理博,MABT134,Anti-VE-cadherin Antibody, clone BV6
merck millipore,默克密理博,AB820,Anti-Apolipoprotein A-II Antibody