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merck millipore,默克密理博,MABT134,Anti-VE-cadherin Antibody, clone BV6
产品名称：Anti-VE-cadherin Antibody, clone BV6
产品型号：MABT134
This Anti-VE-cadherin Antibody, clone BV6 is validated for use in WB, IC, FC, IH(P) for the detection of VE-cadherin.
merck millipore,默克密理博,MABT134,Anti-VE-cadherin Antibody, clone BV6
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H FC, WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MABT134 Replaces MAB1989 描述 Anti-VE-cadherin Antibody, clone BV6 Alternate Names - 7B4 Antigen
- Cadherin-5
背景信息 Human Vascular Endothelial (VE)-cadherin is a calcium-dependent adhesion molecule strictly located at cell-to-cell junctions. VE-cadherin is present in all types of endothelium (veins, arteries, capillary and large vessels). Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins may thus contribute to the sorting of heterogeneous cell types. VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. VE-cadherin also associates with alpha-catenin forming a link to the cytoskeleton. 产品信息 格式 Purified 控制 - HUVEC cell lysate
演示 Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-VE-cadherin Antibody, clone BV6 is validated for use in WB, IC, FC, IH(P) for the detection of VE-cadherin. 主要应用 - Flow Cytometry
- Western Blotting
- Immunocytochemistry
应用说明 Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected VE-cadherin in the cell-cell junctions of HUVEC cells.
Immunohistochemistry Analysis: A 1:10-1:20 dilution of a representative lot was used by an independent laboratory in paraffin-embedded tissue sections. Buffered formalin fixation is recommended with a fixation period of no longer than 12 hours. High heat antigen retrieval in citrate buffer (Cat. No. 21545) is also suggested. Antibody can also be used to label acetone-fixed cryostat sections or cells using a immunoperoxidase staining protocol (eg. IHC Select® Detection Kit, Cat. No. DAB150)
Flow Cytometry Analysis: A representative lot was used in the presence of Ca2+. Note: Use PBS + 2-5mM EDTA for cell detachment.
Western Blot Analysis: Non-reducing conditions may be needed, Ca2+ required in buffer (2-5 mM).生物信息 免疫原品种 HUVEC cells 克隆 BV6 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG2aκ 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001786
Entrez基因汇总 This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. 基因符号 - 7B4
- CDH5
- CD144
纯化方法 Protein G Purified UniProt编号 - P33151
UniProt汇总 FUNCTION: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.
SUBUNIT STRUCTURE: Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1.
SUBCELLULAR LOCATION: Cell junction. Cell membrane; Single-pass type I membrane protein. Note: Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions.产品使用声明 质量保证 Evaluated by Western Blot in HUVEC cell lysate.
Western Blot Anlaysis: A 1:2,000 dilution of this antibody detected VE-cadherin in HUVEC cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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