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  • merck millipore,默克密理博,FCRNC25112,FlowCellect Rodent NSC Characterization Kit (Neural)

  • merck millipore,默克密理博,FCRNC25112,FlowCellect Rodent NSC Characterization Kit (Neural)

    产品名称：FlowCellect Rodent NSC Characterization Kit (Neural)
    产品型号：FCRNC25112
    This FlowCellectRodent Neural Stem Cell Characterization Kit for flow cytometry for Neuronal Differentiation is designed as an easier & quicker way to track neuronal differentiation.

    merck millipore,默克密理博,FCRNC25112,FlowCellect Rodent NSC Characterization Kit (Neural)

  • 产品介绍
  • merck millipore,默克密理博,FCRNC25112,FlowCellect Rodent NSC Characterization Kit (Neural)

    描述
    产品目录编号 FCRNC25112
    商名
    • FlowCellect
    描述 FlowCellect Rodent NSC Characterization Kit (Neural)
    概述 Millipore’s The FlowCellect™Rodent Neural Stem Cell Characterization Kit for Neuronal Differentiation is designed as an easier and quicker way to track neuronal differentiation. The kit will also help to determine the percentage of undifferentiated rat neural stem cells in culture by determining the percentage of cells that express both Sox-2 and Nestin as well as track Beta-III-Tubulin upregulation upon Neuronal differentiation. This quick test will allow researchers to test the quality of their cells in culture as well as see changes in marker expression during a differentiation protocol.

    Sufficient reagents are provided for 25 3-color samples and 5 isotype control tests. The kit includes all optimized fluorescently labeled antibodies and buffers necessary for analysis. To verify antibody specificity, all antibodies were tested against rat neural stem cells as well as rat neurons to verify antibody specificity. A cell strainer is also included to ensure samples are devoid of large cell clumps that can alter cell analysis. Detailed assay instructions are also included to assist in analysis and to ensure the correct cell concentration is obtained for flow cytometric analysis.
    Alternate Names
    • Flow Cellect
    背景信息 Rat Neural Stem cells (rat NSCs) can be maintained in vitro as self renewing multi-potent cells that can be induced to differentiate into astrocytes, neurons, or oligodendrocytes by manipulating culture conditions (1). Therefore, Rat NSCs have the potential of providing an unlimited supply of cells to study differentiation of NSCs to neuronal cell types. Due to the host of neurodegenerative diseases a vast amount of research is generated to study the promise of potential therapeutics involving NSCs.

    Currently, the most utilized method to determine the undifferentiated state of Rat Neural Stem Cells is to analyze the expression of Sox2 and Nestin (3,4). Neural progenitor cells expressing both Sox2 and Nestin have been shown to self-replicate as well as give rise to astrocytes, neurons, and oligodendrocytes under the correct culture conditions (3, 4). Beta-III-Tubulin is a specific component of microtubules found in neuronal axons and cell bodies (5). Upon differentiation to a neuronal lineage, Sox2 and Nestin expression levels decrease while Beta-III-Tubulin expression increases.

    The FlowCellect™ Rat Neural Stem Cell Characterization kit for neurons is designed for cell biology applications. The kit uses an antibody detection method, utilizing fluorescently conjugated primary antibodies to detect Sox2, Nestin and Beta-III-Tubulin. The FlowCellect™ Rodent Neural Stem Cell Characterization Kit for neurons is optimized for flow cytometers with a single blue (488 nm) laser light source.

    The kit is optimized for use on the Guava EasyCyte™ Plus cytometer that requires cell concentrations to be less than 500 cells per microliter. The kit does not include culture media or other materials that should be readily available in a common laboratory setting that routinely performs experiments of the type described here.
    产品信息
    部件
    • 11. Steri-flip cell strainer (60mm): (Catalog # SCNY00060)
    • 1. 10X Wash buffer: (Part No. CS202123) 1 bottle containing 13mL
    • 2. Fixation buffer: (Part No. CS202122) 1 bottle containing 13mL
    • 3. 5X Assay buffer: (Part No. CS202124) 1 bottle containing 55mL
    • 4. 1X Permeabilization Buffer: (Part No. CS202125) 1 bottle containing 13mL
    • 5. 20X IgG2A-FITC isotype control-(Catalog No. CS202584)
    • 6. 20X IgG1-PE isotype control-(Catalog No. CS202573)
    • 7. 20X IgG2a-PE-Cy5 isotype control-(Catalog No. CS202588)
    • 8. 20X Sox-2 FITC-(Catalog No. CS202594)
    • 9. 20X Nestin-PE-(Catalog No. CS202593)
    • 10. 20X Beta-III-Tubulin-PE/CY5-(Catalog No. CS202591)
    检测方法 125IFluorescent
    应用
    应用 This FlowCellectRodent Neural Stem Cell Characterization Kit for flow cytometry for Neuronal Differentiation is designed as an easier & quicker way to track neuronal differentiation.
    主要应用
    • Flow Cytometry
    生物信息
    宿主 Mouse
    品种反应性 Rat
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM030773
    • NM_003106.2
    • NM_006617.1
    基因符号
    • TUBB3
    • SOX2
    • NES
    UniProt编号
    • Q13509
    • P48431
    • P48681
    产品使用声明
    质量保证 Lot to lot in-process testing on all kit components
    使用声明
    • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 4-8°C; 6 Months
    包装信息
    数量 25 Tests / Kit

    merck millipore,默克密理博,FCRNC25112,FlowCellect Rodent NSC Characterization Kit (Neural)

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