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品种反应性 主要应用 宿主 格式 抗体类型 M WB, ELISA Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB15504 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Klotho Antibody 背景信息 Animal models of human aging, which display the characteristic diseases associated with aging, provide insight into the cause of such diseases. Recently a transgenic mouse model, called Klotho, with several premature aging phenotypes has been described. They display premature aging phenotypes such as Osteoporosis, age related skin changes, ectopic calcifications, atrophy of genital organs and thymus, emphysema and short life span (Kuro-o et al., 1997). KL protein associated with the Klotho mutation is a 1014 amino acid long peptide. It has a putative signal sequence at its N-terminus and a single transmembrane domain near its C-terminus, which is postulated to anchor it to the membrane (Martin & Mian, 1997). The extracellular domain has two internal repeats, KL-1 and KL-2 which have 20-40% sequence identity to bacterial b-glucosidase of plants and animals and mammalian lactase glycosylceramidase suggesting a role for Klotho in sphingolipid metabolism (Martin & Mian, 1997). kl gene expression was observed to be tissue specific. Improvement of systemic aging phenotypes in kl/kl mice occurs even when the exogenous expression was limited to some organs, suggesting that KL associated aging is regulated through a humoral signaling pathway. KL has been reported to be localized on the cell surface when expressed on Cho cells (Kuro-o et al., 1997). Human kl cDNA is expected to encode a protein of 1012 amino acids and has 86% homology to mouse kl. 产品信息 格式 Affinity Purified 演示 Liquid in PBS with 0.1% BSA. 应用 应用 Detect Klotho using this Anti-Klotho Antibody validated for use in ELISA & WB. 主要应用 - Western Blotting
- ELISA
应用说明 Western blot: 1-10 µg/mL using ECL.
Immunohistochemistry: Not tested. It is recommended that the antibody be tried at 2-20 µg/mL
ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide/well.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Synthetic peptide near the C-terminus of mouse Klotho. 宿主 Rabbit 特异性 Klotho 品种反应性 Mouse Species Reactivity Note Other species have not yet been tested. The immunogen sequence is 82% conserved in human and 76% in rat klotho. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004795.2
- NM_153683.2
Entrez基因汇总 This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. 基因符号 - EC 3.2.1.31 [Contains: Klotho peptide].
- KL
- klotho
纯化方法 Immunoaffinity purified UniProt编号 - Q9UEF7
UniProt汇总 FUNCTION: SwissProt: Q9UEF7 # The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling (By similarity).
SIZE: 1012 amino acids; 116181 Da
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. Note=Isoform 1 shedding leads to a soluble peptide. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.
DOMAIN:SwissProt: Q9UEF7 Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 239, and the second one lacks the essential Glu active site residue at position 872.
PTM: N-glycosylated (By similarity).DISEASE:SwissProt: Q9UEF7 # Defects in KL may be a cause of chronic renal failure complications.
SIMILARITY: Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µg
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