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merck millipore,默克密理博,04-1565,Anti-NSD1 Antibody, clone 1NW-1A10
产品名称：Anti-NSD1 Antibody, clone 1NW-1A10
产品型号：04-1565
Anti-NSD1 Antibody, clone 1NW-1A10 is a mouse monoclonal antibody for detection of NSD1, also known as &rogen receptor-associated coregulator 267, Lysine N-methyltransferase 3B, has been validated in More>>
merck millipore,默克密理博,04-1565,Anti-NSD1 Antibody, clone 1NW-1A10
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, R ICC, WB M Ascites Monoclonal Antibody 描述 产品目录编号 04-1565 描述 Anti-NSD1 Antibody, clone 1NW-1A10 Alternate Names - nuclear receptor binding SET domain protein 1
- Androgen receptor-associated coregulator 267
- Lysine N-methyltransferase 3B
- NR-binding SET domain-containing protein
- Nuclear receptor-binding SET domain-containing protein 1
- Sotos syndrome
背景信息 NSD1 (also known as KMT3B) is a histone methyltransferase shown to methylate lysine 36 of histone H3 and lysine 20 of histone H4. NSD1 is a described as an intermediary transcription factor and depending on its cellular context, it is able to either negatively or positively influence transcription. Expression of this gene has been described in skeletal muscles, kidneys, adult and fetal brain, thymus and spleen. Heterozygous mutations and deletions of the NSD1 gene are associated with Sotos Syndrome, an autosomal dominant condition characterized by macrocephaly, tall stature, facial elongation, accelerated bone aging, and mental retardation. 产品信息 格式 Ascites 控制 - PC12 cell lysate
演示 Unpurified mouse monoclonal IgG1κ in preservative-free ascites. 应用 应用 Anti-NSD1 Antibody, clone 1NW-1A10 is a mouse monoclonal antibody for detection of NSD1, also known as &rogen receptor-associated coregulator 267, Lysine N-methyltransferase 3B, has been validated in WB. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Used for immunohistochemistry on paraffin sections in Visser et al., (2012).
Used for fluorescent immunocytochemistry in Huang et al.,(1998).生物信息 免疫原品种 Recombinant protein corresponding to mouse NSD1. 表位 Unknown 克隆 clone 1NW-1A10 宿主 Mouse 特异性 This antibody recognizes NSD1. 同种型 IgG1κ 品种反应性 MouseRat Species Reactivity Note Demonstrated to react with rat. Predicted to react with mouse based on 100% sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_032765.3
基因符号 - STO
- NSD1
- ARA267
- H3-K36-HMTase
- H4-K20-HMTase
- KMT3B
- SOTOS
纯化方法 Unpurified UniProt编号 - O88491
UniProt汇总 FUNCTION: Histone methyltransferase. Preferentially methylates 'Lys-36' of histone H3 and 'Lys-20' of histone H4 (in vitro). Transcriptional intermediary factor capable of negatively influencing transcription. May also positively influence transcription. Essential for early post-implantation development.
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.
SUBUNIT STRUCTURE: Interacts with AR DNA- and ligand-binding domains by similarity. Interacts with the ligand-binding domains of RARA and THRA in the absence of ligand; in the presence of ligand the interaction is severely disrupted but some binding still occurs. Interacts with the ligand-binding domains of RXRA and ESRRA only in the presence of ligand.
SUBCELLULAR LOCATION: Nucleus
TISSUE SPECIFICITY: Expressed in the embryo and the outer region of the uterine decidua at early post-implantation E5.5 stage. Uniformly expressed in embryonic and extraembryonic tissues during gastrulation stage E7.5. Expressed differentially after stage 14.5 with highest expression in proliferating cells. Enriched in the telencephalic region of the brain, spinal cord, intestinal crypt, tooth buds, thymus and salivary glands at stage E16.5. Also expressed in the ossification region of developing bones and in the periosteum.
DOMAIN: Contains 2 adjacent but distinct nuclear receptor interaction domains (NID+L and NID-L) to which nuclear receptors bind differentially in the presence and absence of ligand respectively.
SEQUENCE SIMILARITIES: Belongs to the histone-lysine methyltransferase family.
Contains 1 AWS domain.
Contains 4 PHD-type zinc fingers.
Contains 1 post-SET domain.
Contains 1 PWWP domain.
Contains 1 SET domain.产品使用声明 质量保证 Evaluated by Western Blot in PC12 cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected NSD1 on 10 µg of PC12 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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