merck millipore,默克密理愽
  • merck millipore,默克密理博,AB1513P,Anti-Brain Derived Neurotrophic Factor Antibody

  • merck millipore,默克密理博,AB1513P,Anti-Brain Derived Neurotrophic Factor Antibody

    产品名称：Anti-Brain Derived Neurotrophic Factor Antibody
    产品型号：AB1513P
    Anti-Brain Derived Neurotrophic Factor Antibody detects level of Brain Derived Neurotrophic Factor & has been published & validated for use in ELISA, IH, FUNC & WB.

    merck millipore,默克密理博,AB1513P,Anti-Brain Derived Neurotrophic Factor Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB1513P,Anti-Brain Derived Neurotrophic Factor Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, Ch, HWB, ELISA, IHCShPurifiedPolyclonal Antibody
    描述
    产品目录编号 AB1513P
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Brain Derived Neurotrophic Factor Antibody
    Alternate Names
    • BDNF
    产品信息
    格式 Purified
    控制
    • Brain tissue
    演示 Protein G Purified immunoglobulin. Lyophilized from PBS, no preservatives. Reconstitute with 500 μL of sterile distilled water.
    应用
    应用 Anti-Brain Derived Neurotrophic Factor Antibody detects level of Brain Derived Neurotrophic Factor & has been published & validated for use in ELISA, IH, FUNC & WB.
    主要应用
    • Western Blotting
    • ELISA
    • Immunohistochemistry
    应用说明 Immunohistochemistry: 1-5 μg/mL (see suggested protocol).

    Immunoblotting: 1-5 μg/mL we recommend preparing the BDNF samples for western blot by the method of Semba-Katoh, R et.al (J. Neurochem. 69(1):34-42, 1997) because BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4°C. BDNF migrates as ~12-18kDa monomer, with a 28-30kDa homodimer in most samples.

    ELISA: 1-5 μg/mL

    Inhibition of biological activity in vitro: Complete inhibition of BDNF activity on embryonic chick sensory neurons occurs at 1-5 μg/mL. Biological inhibition protocols can be found in Finn et al. (J. Neurocytol. 15:169-176, 1986).
    BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.

    Use neat for in vivo studies.

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Recombinant Human Brain Derived Neurotrophic Factor (BDNF).
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Sheep
    特异性 Recognizes Brain Derived Neurotrophic Factor (BDNF). Neutralizes BDNF, but not other neurotrophins. By one site ELISA, less than 1% cross-reactivity against mouse NGF, recombinant human NT3 or neurotrophin 4. The antibody was tested on human and rat derived samples, but based on sequence similarity it is expected to react with mouse and other mammalian species. The peptide sequences of human, rat and mouse BDNF are identical.
    品种反应性 RatChickenHuman
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_170735.4
    • NM_001709.3
    • NM_170731.3
    • NM_170732.3
    • NM_170733.2
    • NM_170734.2
    Entrez基因汇总 The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene, but the full-length nature of only some could be determined.
    基因符号
    • MGC34632
    • BDNF
    • Abrineurin
    • neurotrophin
    纯化方法 Protein G purfied
    UniProt编号
    • P23560
    UniProt汇总 FUNCTION: SwissProt: P23560 # Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
    SIZE: 247 amino acids; 27818 Da
    SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB.
    SUBCELLULAR LOCATION: Secreted.
    PTM: The propeptide is N-glycosylated and glycosulfated. & Converted into mature BDNF by plasmin (PLG) (By similarity).
    DISEASE: SwissProt: P23560 # Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
    SIMILARITY: SwissProt: P23560 ## Belongs to the NGF-beta family.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Glycerol (1:1) can be added for additional stability. Avoid repeated freeze/thaw cycles.
    包装信息
    数量 500 µg

    merck millipore,默克密理博,AB1513P,Anti-Brain Derived Neurotrophic Factor Antibody

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