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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB125 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Serotonin Antibody Alternate Names - 5-HT
产品信息 格式 Serum 演示 Rabbit antiserum. Liquid with 0.05% sodium azide. 应用 应用 Anti-Serotonin Antibody detects level of Serotonin & has been published & validated for use in IH. 主要应用 - Immunohistochemistry
应用说明 Immunohistochemistry: 1:1,000-1:2,500 by ABC or enhanced detection. Glutaraldehyde fixation is required. Antibody will not react with non-glutaraldehyde treated tissues. Blocking should be with serum or BSA containing 0.1-0.2% triton X-100; remove all blocking an detergents during primary antibody incubation. Dilute only in PBS or TBS for best results. For fluorescent detection antibody dilution must be reduced, but it is not recommended because of glutaraldehyde autofluorescence.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 5-Hydroxytryptamine-glutaraldehyde-Poly-lysine 宿主 Rabbit 特异性 Serotonin (5-Hydroxytryptamine)
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity
5-Hydroxytryptamine-G-PL 1
5-Methoxytryptamine-G-PL 1/25
Tryptamine-G-PL 1/60
5-Hydroxytryptophan-G-PL 1/175
5-Hydroxytryptamine 1/1150
Tryptophan-G-PL 1/3400
5-Methoxytryptophan-G-PL 1/19800
The antisera was also tested for specificity using the free-floating PAP technique on rat
raphe nuclei.
Abbreviations:
(G) Glutaraldehyde
(=) Non-reduced conjugate
(PL) Poly-lysine品种反应性 Rat 抗体类型 Polyclonal Antibody 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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