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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB10620 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Cdc4 Antibody 背景信息 The F-box protein family is characterized by an approximately 40 amino acid motif, the F-box. F-box proteins are divided into 3 classes: FBWs containing WD-40 domains, FBLs containing leucine-rich repeats, and FBXs containing either different protein-protein interaction modules or no recognizable motifs. They constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination.
Cdc4 is one member of this family, which is also known as AGO, FBW7, FBX30 or SEL10. CDC4 binds directly to cyclin E and targets cyclin E for ubiquitin-mediated degradation. Cdc4 proteins of amino acid lengths varying from 553 aa to 707 aa in length have been reported. Cdc4 is an inhibitor of Notch signaling that targets Notch for ubiquitin-mediated degradation after a nuclear phosphorylation event.5,6 Cdc4 interacts with presenilin 1, facilitates its ubiquitination, and alters A-beta peptide production. Thus, it may be important for Alzheimer's disease. Mutations of the CDC4 gene are detected in ovarian and breast cancer cell lines and the CDC4 gene may also be involved in the pathogenesis of human pancreatic and endometrial cancers.产品信息 格式 Affinity Purified 演示 This polyclonal antibody is supplied in 400 μL of phosphate buffered saline (pH 7.4) containing 0.1% sodium azide. 应用 应用 Anti-Cdc4 Antibody is an antibody against Cdc4 for use in WB & IP. 主要应用 - Western Blotting
应用说明 Western Blotting: 1-3 μg/mL
Immunoprecipitation: 7 µg/reaction
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Synthetic peptide derived from an internal region of the human and mouse Cdc4 (archipelago (AGO), F-box/WD-repeat protein 7, F-box and WD-40 domain protein 7, F-box protein FBW7, FBX30 and SEL-10). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes CDC4. 品种反应性 Human Species Reactivity Note Reactivity has been confirmed with human T47D, U2-OS, MCF-7, HepG2 and HeLa cell lysates. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_033632.2
- NM_001013415.1
- NM_018315.4
Entrez基因汇总 This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Three transcript variants encoding three different isoforms have been found for this gene. 基因符号 - hCdc4
- FBXW7
- DKFZp686F23254
- AGO
- FBXW6
- SEL-10
- hAgo
- CDC4
- FBX30
- FLJ11071
- SEL10
- FBW7
纯化方法 Immunoaffinity purified UniProt编号 - Q969H0
UniProt汇总 FUNCTION: SwissProt: Q969H0 # Recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. Involved in the degradation of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.
SIZE: 707 amino acids; 79663 Da
SUBUNIT: Part of a SCF complex consisting of CUL1, RBX1, SKP1 and FBXW7. Interacts with PSEN1, cyclin E, NOTCH1 NICD, NOTCH4 NICD and SKP1A.
SUBCELLULAR LOCATION: Nucleus (By similarity).
TISSUE SPECIFICITY: Isoform 1 is widely expressed. Isoform 4 is expressed in brain.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
DISEASE:SwissProt: Q969H0 # Defects in FBXW7 may be a cause of breast cancer.
SIMILARITY: SwissProt: Q969H0 ## Contains 1 F-box domain. & Contains 7 WD repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at -20°C in undiluted aliquots for 1 year from date of shipment. 包装信息 数量 100 µg
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