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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB1031 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Aggrecan Antibody Alternate Names - large aggregating proteoglycan
- Cartilage-specific proteoglycan core protein
- aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122)
- aggrecan proteoglycan
- antigen identified by monoclonal antibody A0122)
背景信息 Aggregating chondroitin sulfate proteoglycan or aggrecan, is one of the most studied proteoglycans. Many individual monomers of aggrecan bind to hyaluronic acid to form an aggregate, the monomer is known as aggrecan. The aggregates are comprised of up to 100 monomers attached to a single chain of hyaluronic acid. The high content of negatively charged polysaccharide chains contributes to the osmotic swelling of cartilage and in combination with intact collagen fibers results in articular cartilage resistance to compressive forces and high tensile strength. Aggrecan is abundant in articular cartilage and compromises 10% of its dry weight. 产品信息 格式 Affinity Purified 控制 - Cartilage, neural tube, and brain tissue.
演示 Rabbit polyclonal antigen affinity purified antibody in PBS. Anti-GST has not been adsorbed. Liquid.
Note: Aggrecan is present in articular cartilage, brain, heart, and other tissues.应用 应用 Anti-Aggrecan Antibody is an antibody against Aggrecan for use in IF, IH(P), IP & WB. 主要应用 - Western Blotting
应用说明 Immunohistochemistry(Paraffin):
A previous lot of this antibody was used at 10 μg/mL. See note under Protocol regarding triton X-100 treatments.
Immunoprecipitation:
A previous lot of this antibody was used at 5 μg/ 500 μL of cell membrane extract in PBS with inhibitors and >0.5% triton X-100.生物信息 免疫原品种 GST fusion protein containing amino acids 1177-1326 of mouse aggrecan. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 AB1031 is specific for mouse aggrecan; other species have not been tested. 品种反应性 Mouse Species Reactivity Note Mouse. Expected to cross react with Human and rat. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001126
Entrez基因汇总 This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. 基因符号 - CSPGCP
- ACAN
- MSK16
- SEDK
- CSPCP
- AGCAN
- AGC1
- CSPG1
纯化方法 Immunoaffinity purified UniProt编号 - P16112
UniProt汇总 FUNCTION: SwissProt: P16112 # This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.SIZE: 2415 amino acids; 250193 DaSUBUNIT: Interacts with FBLN1 (By similarity).SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).TISSUE SPECIFICITY: Restricted to cartilages.DEVELOPMENTAL STAGE: Expression was detected in chondrocytes throughout the developing skeleton.DOMAIN: SwissProt: P16112 Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.
PTM: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.DISEASE: SwissProt: P16112 # Defects in AGC1 are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK) [MIM:608361]. Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy.SIMILARITY: Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 1 EGF-like domain. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 4 Link domains. & Contains 1 Sushi (CCP/SCR) domain.产品使用声明 质量保证 Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of this lot detected AGGRECAN on 10 μg of Mouse Brain lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 50 µg
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