merck millipore,默克密理愽
  • merck millipore,默克密理博,A-003-E,Poly-D-Lysine solution, 1.0 mg/mL

  • merck millipore,默克密理博,A-003-E,Poly-D-Lysine solution, 1.0 mg/mL

    产品名称：Poly-D-Lysine solution, 1.0 mg/mL
    产品型号：A-003-E
    The Poly-D-Lysine solution promotes the adhesion of tissues/sections to the culture vessel.

    merck millipore,默克密理博,A-003-E,Poly-D-Lysine solution, 1.0 mg/mL

  • 产品介绍
  • merck millipore,默克密理博,A-003-E,Poly-D-Lysine solution, 1.0 mg/mL

    重要规格表

    主要应用
    CULT
    描述
    产品目录编号 A-003-E
    商名
    • Specialty Media
    描述 Poly-D-Lysine solution, 1.0 mg/mL
    概述 Poly-D-Lysine promotes the adhesion of tissues/sections to the culture vessel. The polycationic property of the molecule allows interaction with anionic sites on the tissue/cells promoting effective attachment to the growing surface. The molecular weight of Poly-D-Lysine is >300,000 Da.
    Alternate Names
    • poly lysine
    产品信息
    演示 Liquid. 20ml in sterile water.
    应用
    应用 The Poly-D-Lysine solution promotes the adhesion of tissues/sections to the culture vessel.
    主要应用
    • Cell Culture
    应用说明 The level of coating will vary for different species and types of cells. In general final coating concentrations from 3-10μg/cm(2) of surface area will typically supply sufficent coatings for most cell types.



    Surface area of plates: (pi)(Radius squared) in cm(2)



    In partical terms working dilutions of 50μg/mL to 100μg/mL are usually adequate.



    Dilute poly-D-lysine in PBS, water, or 0.1M borate buffer {prepared by adding 1.24 g boric acid and 1.9 g sodium tetraborate in 400 ml water, pH 8.5} to final working concentration.

    Plates or flasks are coated for 3 hours to overnight, and up to 3 days if stored at 4C. Best results are usually with just over night coatings.

    Remove poly-d-lysine solution and rinse with sterile PBS or water 1-2X. Do not scrape the bottom of the coated plates or flasks.

    Use plates immediately or store with PBS for up to 5 days.



    Poly-D-lysine coating methods will vary slightly from laboratory to laboratory, please use the method as a general guide.
    生物信息
    品种反应性 All
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Store at -20C for upto 18 months.
    包装信息
    数量 20 mL

    merck millipore,默克密理博,A-003-E,Poly-D-Lysine solution, 1.0 mg/mL

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