merck millipore,默克密理博,71546,BacMagic™ Transfection Kit

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Merck Millipore 默克密理愽

merck millipore,默克密理博,71546,BacMagic™ Transfection Kit - Novagen
产品名称：BacMagic™ Transfection Kit - Novagen
产品型号：71546

merck millipore,默克密理博,71546,BacMagic™ Transfection Kit - Novagen

merck millipore,默克密理博,71546,BacMagic™ Transfection Kit - Novagen

描述
概述	The BacMagic™-1 Transfection Kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. BacMagic DNA is an AcNPV genome with a portion of the essential open reading frame (ORF) 1629 deleted, preventing nonrecombinant virus from replicating in insect cells. The target coding sequence in a compatible transfer plasmid, such as pIEx/Bac™, pBAC™, or pTriEx™ vectors, is cotransfected with BacMagic DNA into insect cells. Homologous recombination within the cells restores the function of the viral ORF1629. Only recombinant baculovirus can replicate, producing a homogeneous population of recombinants. The BacMagic™-1 Transfection Kit contains the BacMagic™-1 DNA, Insect GeneJuice^® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency. In addition, it contains Sf9 cells and BacVector^® Medium, for optimal transfection performance.
产品目录编号	71546
品牌系列	Novagen®
应用数据	Figure 1. Construction of baculovirus recombinants with three BacMagic systems. Figure 2. Streamlined baculovirus method

产品信息
部件

储存和货运信息
货运代码	Multiple Storage Conditions
毒性	Multiple Toxicity Values, refer to MSDS
储存	Multiple storage requirements
无需冷冻	No

merck millipore,默克密理博,71546,BacMagic™ Transfection Kit - Novagen

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