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描述 概述 NovaTaq™ Hot Start DNA Polymerase is a chemically modified form of Taq DNA polymerase that is inactive at ambient temperature. The enzyme provides improved specificity when compared to standard Taq DNA polymerase and can minimize the generation of nonspecific amplification products, such as primer-dimers and misprimed products. The enzyme must be activated by heat treatment (7–10 min at 95°C), after which thermal cycling can proceed. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with the Novagen Perfectly Blunt®, AccepTor™, and LIC Vector Kits.
Source Recombinant Thermus aquaticus DNA polymerase expressed in E. coli Concentration 5.0 U/µl Endonuclease None detected Exonuclease None detected Amplification effiency Functional PCR Storage –20°C 产品目录编号 71091 品牌系列 Novagen®
特点及优点 - Higher PCR specificity and yield
- Improved low-copy target amplification
- Ambient temperature setup compatible with automation
- Target amplification of up to 5 kbp
- Ideal for quantitative and high-throughput PCR applications
产品信息 部件 声明 Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA. 储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 -20°C
无需冷冻 No
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