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描述 概述 Proteinase K is a highly active 28,904-Da serine protease isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA. Its activity is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C). The recommended working concentration is 50-100 µg/ml for protein removal and enzyme inactivation, and up to 2 mg/ml for tissue treatment. The Proteinase K, Lyophilized powder can be prepared as a 20 mg/ml stock solution in water and stored in aliquots at -20°C. The enzyme is also available as a ready-to-use concentrated stock solution (600 mAU/ml) that is convenient for routine use in most applications. 1 mg of Proteinase K is the equivalent of 30 mAU (AU = Anson unit). The Novagen Proteinase K products are free of detectable DNase and RNase 产品目录编号 71049 品牌系列 Novagen®
理化信息 污染物 Free of detectable DNase and RNase. 储存和货运信息 货运代码 Shipped with Blue Ice or with Dry Ice
毒性 Harmful
储存 -20°C
无需冷冻 No
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