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merck millipore,默克密理博,05-373,Anti-Cyclin B1 Antibody, clone GNS3 (8A5D12)
产品名称：Anti-Cyclin B1 Antibody, clone GNS3 (8A5D12)
产品型号：05-373
Anti-Cyclin B1 Antibody, clone GNS3 (8A5D12) is a Mouse Monoclonal Antibody for detection of Cyclin B1 also known as G2/mitotic-specific cyclin B1 & has been validated in IP & WB.
merck millipore,默克密理博,05-373,Anti-Cyclin B1 Antibody, clone GNS3 (8A5D12)
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB M Purified Monoclonal Antibody 描述 产品目录编号 05-373 Replaces 04-220 品牌系列 Upstate
商名 - Upstate
描述 Anti-Cyclin B1 Antibody, clone GNS3 (8A5D12) Alternate Names - cyclin B1
- G2/mitotic-specific cyclin B1
背景信息 Regulation of cell cycle progression in eukaryotic cells depends on the expression of cyclin proteins. These proteins are the regulatory subunits of the cell cycle-dependent kinases that are responsible for the phosphorylation of several cellular targets. The B cyclins, when combined with p34cdc2, a catalytic subunit of the cell cycle dependent kinase, form an enzyme that is active at the transition to the G2 phase, attaining maximum level at M phase. At that phase, the complex associates with condensed chromosomes in prophase and metaphase and is degraded at the metaphase/anaphase transition.
Anti-Cyclin B1 may be used for the detection of cyclin B1 expression and for measuring relative differences in cyclin B1 level as a function of cell cycle phase.产品信息 格式 Purified 控制 - A431 cells
Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
演示 Protein G purified mouse IgGs in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen at -20°C. 应用 应用 Anti-Cyclin B1 Antibody, clone GNS3 (8A5D12) is a Mouse Monoclonal Antibody for detection of Cyclin B1 also known as G2/mitotic-specific cyclin B1 & has been validated in IP & WB. 主要应用 - Western Blotting
应用说明 Immunoprecipitation:
2 μg of a previous lot immunoprecipitated human cyclin B1 and cdc2 kinase from 500 μg of A431 RIPA lysate as determined by a subsequent immunoblot of the immunoprecipitate using anti-cdk1/cdc2 (PSTAIR), (Catalog # 06-923).生物信息 免疫原品种 Recombinant human cyclin B1 克隆 GNS3 (8A5D12) 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody is specific for human cyclin B1, Mr 58 kDa. Does not cross-react with cyclin A or cyclin D. 同种型 mixed IgGs 品种反应性 MouseHuman Species Reactivity Note In addition to human, weak species cross-reactivity was observed with mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_031966
Entrez基因汇总 The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. 基因符号 - CCNB
- CCNB1
纯化方法 Protein G chromatography UniProt编号 - P14635
UniProt汇总 FUNCTION: SwissProt: P14635 # Essential for the control of the cell cycle at the G2/M (mitosis) transition.
SIZE: 433 amino acids; 48337 Da
SUBUNIT: Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Centrosome.
DEVELOPMENTAL STAGE: Accumulates steadily during G2 and is abruptly destroyed at mitosis.
PTM: Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
SIMILARITY: SwissProt: P14635 ## Belongs to the cyclin family. Cyclin AB subfamily.产品使用声明 质量保证 Routinely evaluated by western blot analysis on RIPA lysate from human A431 carcinoma cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected cyclin B1 in RIPA lysates from human A431 carcinoma cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 200 µg
上一件merck millipore产品：merck millipore,默克密理博,17-658,ChIPAb+ Acetyl-Histone H3 (Lys9) Purified - ChIP Validated Antibody and Primer Set
下一件merck millipore产品：merck millipore,默克密理博,04-337,Anti-EGFR Antibody, clone E114, rabbit monoclonal
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