merck millipore,默克密理博,69830,Human Ig-Primer Set

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Merck Millipore 默克密理愽

描述
概述	The Ig-Primer Sets are designed for amplification of immunoglobulin (Ig) variable region cDNAs from human and mouse sources. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR (Larrick 1989, Jones 1991), after which the products may be sequenced directly or cloned. This powerful method enables rapid, precise analysis of hybridomas, myeloma cell progression and heterogeneity, idiotype networks, B-cell clonal ontogeny, and phylogenetic relationships. In practice, total RNA is isolated from a B-cell source, such as peripheral blood, lymph node(s), spleen, or hybridoma, and first-strand cDNA is prepared using reverse transcriptase. The primer sets are then used in PCR to specifically amplify the variable regions of light- and heavy-chain cDNAs.
概述	Please call Technical Service if you need more information regarding individual Ig-Primer Sets.
产品目录编号	69830
品牌系列	Novagen®

参考
参考	Larrick, J.W., et al. 1989. Biochem. Biophys. Res. Comm. 160, 1250. Jones, S.T. and Bendig, M.M. 1991. Biotechnology 9, 88.

产品信息
部件

储存和货运信息
货运代码	Shipped with Blue Ice or with Dry Ice
毒性	Standard Handling
储存	-20°C
避免冻结 / 解冻	Avoid freeze/thaw
无需冷冻	No

merck millipore,默克密理博,69830,Human Ig-Primer Set

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