merck millipore,默克密理愽
  • merck millipore,默克密理博,69080,pT7Blue-2 T-Vector - Novagen

  • merck millipore,默克密理博,69080,pT7Blue-2 T-Vector - Novagen

    产品名称：pT7Blue-2 T-Vector - Novagen
    产品型号：69080


    merck millipore,默克密理博,69080,pT7Blue-2 T-Vector - Novagen

  • 产品介绍
  • merck millipore,默克密理博,69080,pT7Blue-2 T-Vector - Novagen

    描述
    概述 The pT7Blue-2 T-Vector is designed for simplified cloning of PCR products. Many thermostable DNA polymerases, such as Taq DNA polymerase, leave single 3' A-nucleotide overhangs on their reaction products. These molecules can be ligated directly to a vector containing compatible single T-nucleotide overhangs. The pT7Blue-2 vector has been specifically constructed for this application and has been prepared for T-cloning by digestion with EcoRV followed by the addition of single 3' dT residues at each end. The T-Vector is provided ready to ligate with DNA amplified with any DNA polymerase that leaves single 3' dA overhangs. Target inserts having other types of ends, such as reaction products of proofreading polymerase or restriction fragments, can be easily prepared for T-cloning with the Single dA™ Tailing Kit (Cat. No. 69282-3).

    The pT7Blue-2 Vector is a versatile plasmid featuring the 43-bp Xenopus β-globin 5'-UTR for enhancement of translation in rabbit reticulocyte lysates and frog oocytes. Inserts cloned in-frame with the lacZ α-peptide coding sequence can also be expressed in E. coli from the lac promoter. The vector has many additional features that make it an excellent choice for a variety of applications.
    产品目录编号 69080
    品牌系列 Novagen®
    产品信息
    部件
    储存和货运信息
    货运代码 Shipped with Blue Ice or with Dry Ice
    毒性 Standard Handling
    储存 -20°C
    无需冷冻 No

    merck millipore,默克密理博,69080,pT7Blue-2 T-Vector - Novagen

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