merck millipore,默克密理愽
  • merck millipore,默克密理博,69042,B834(DE3)pLysS Competent Cells - Novagen

  • merck millipore,默克密理博,69042,B834(DE3)pLysS Competent Cells - Novagen

    产品名称：B834(DE3)pLysS Competent Cells - Novagen
    产品型号：69042
    B834 host strains are methionine auxotrophs that allows high specific activity labeling of target proteins with 35S-methionine. T7 lysozyme expression suppresses basal T7 expression.

    merck millipore,默克密理博,69042,B834(DE3)pLysS Competent Cells - Novagen

  • 产品介绍
  • merck millipore,默克密理博,69042,B834(DE3)pLysS Competent Cells - Novagen

    描述
    概述 B834 is the parental strain for BL21 (1). These protease-deficient hosts are methionine auxotrophs and allow high specific-activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography (2).

    DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

    pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
    Genotype: F- ompT hsdSB(rB- mB-) gal dcm met (DE3) pLysS (CamR)
    产品目录编号 69042
    品牌系列 Novagen®
    参考
    参考 1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133.

    2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.
    产品信息
    部件
    保证高效 Guaranteed efficiency: > 2 x 10⁶ cfu/μg
    储存和货运信息
    货运代码 Dry Ice Only
    毒性 Multiple Toxicity Values, refer to MSDS
    储存 ≤ -70°C
    避免冻结 / 解冻 Avoid freeze/thaw
    无需冷冻 No
    布鲁克黑文许可证 This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

    merck millipore,默克密理博,69042,B834(DE3)pLysS Competent Cells - Novagen

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