merck millipore,默克密理博,S7450,OxyIHC™ Oxidative Stress Detection Kit

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merck millipore,默克密理博,S7450,OxyIHC™ Oxidative Stress Detection Kit
产品名称：OxyIHC™ Oxidative Stress Detection Kit
产品型号：S7450
The OxyIHC Oxidative Stress Detection Kit contains the chemical & immunological reagents necessary to detect protein oxidation in various tissues from a variety of organs & animal species.

merck millipore,默克密理博,S7450,OxyIHC™ Oxidative Stress Detection Kit

产品介绍

merck millipore,默克密理博,S7450,OxyIHC™ Oxidative Stress Detection Kit

重要规格表

主要应用	检测方法
IHC	Chromogenic

描述
产品目录编号	S7450
商名	OxyIHC
描述	OxyIHC™ Oxidative Stress Detection Kit
概述	The OxyIHC™ Oxidative Stress Detection Kit (S7450) contains the chemical and immunological reagents necessary to detect protein oxidation in various tissues from a variety of organs and animal species. The OxyIHC test method involves chemical derivatization of protein carbonyl groups with 2,4- dinitrophenylhydrazine (DNPH). This chemical reaction results in proteins being covalently coupled to DNP at their carbonyl sites. The DNP-derivatized proteins are then detected using an antibody that specifically binds to the DNP moiety. Subsequent incubation with biotin conjugated secondary antibody, streptavidin conjugated HRP, and development using a 3,3’ diaminobenzidine (DAB) staining allows immunohistochemical detection of protein oxidation.
背景信息	Reactive oxygen species (ROS) have been implicated in pathological processes including cancer, apoptosis, aging, neurodegenerative diseases, chronic inflammatory diseases, pulmonary diseases, and cardiovascular diseases (for reviews, see ref. 1-4). Living organisms are continually exposed to potentially harmful oxygen free radicals that are generated thru normal cellular functions as well as from environmental factors (1, 5-7). Yet, these reactive molecules are mitigated by the presence of antioxidants and therefore organisms exist with a delicate balance between oxidants and antioxidants (7-9). During times of oxidative stress there is an imbalance in favor of the oxidants. The end result of which is that free radicals are able to attack and modify sub-cellular components including nucleic acids, lipids and proteins (7, 10-12). Proteins are one of the major targets of reactive species. Oxidation of proteins modifies the side chains of methionine, histidine, and tyrosine and forms cysteine disulfide bonds (16-19). Metal catalyzed oxidation of proteins introduces carbonyl groups (aldehydes and ketones) at lysine, arginine, proline or threonine residues in a site-specific manner (16, 20-22). The oxidative modification of proteins can modulate biochemical characteristics of proteins such as enzymatic activity (21-23), DNA binding activities of transcription factors (24-26), and the susceptibility to proteolytic degradation (12, 25-28). While a relationship between protein oxidation and aging has been suggested (29-31), little is known about the importance of oxidative modification of individual proteins in the pathophysiology of free radical mediated processes. Not surprisingly, carbonyl modification of proteins has become a key biomarker for the identification of oxidative stress.

产品信息
部件	Derivatization Control Solution, One ready to use 12.5 mL bottle 10X Wash Buffer, One 25 mL bottle 10X Antigen Retrieval Buffer, One 1.25 mL bottle 5X Blocking Buffer, One 2.5 mL bottle Primary Antibody, One 125 mL vial DAB-A, One 500 mL vial DAB-B, One 12 mL bottle Antibody Diluent, One ready to use 12.5 mL bottle Biotinylated Secondary Antibody, One ready to use 12.5 mL bottle Streptavidin conjugated HRP, One ready to use 12.5 mL bottle Hematoxylin, One ready to use 12.5 mL bottle DNPH Solution, One ready to use 12.5 mL bottle
检测方法	Chromogenic

产品信息

部件

Derivatization Control Solution, One ready to use 12.5 mL bottle
10X Wash Buffer, One 25 mL bottle
10X Antigen Retrieval Buffer, One 1.25 mL bottle
5X Blocking Buffer, One 2.5 mL bottle
Primary Antibody, One 125 mL vial
DAB-A, One 500 mL vial
DAB-B, One 12 mL bottle
Antibody Diluent, One ready to use 12.5 mL bottle
Biotinylated Secondary Antibody, One ready to use 12.5 mL bottle
Streptavidin conjugated HRP, One ready to use 12.5 mL bottle
Hematoxylin, One ready to use 12.5 mL bottle
DNPH Solution, One ready to use 12.5 mL bottle

检测方法

Chromogenic

应用
应用	The OxyIHC Oxidative Stress Detection Kit contains the chemical & immunological reagents necessary to detect protein oxidation in various tissues from a variety of organs & animal species.
主要应用	Immunohistochemistry

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	The OxyIHC kit components should be stored at 2-8ºC. Kit components are stable for 6 months from the date of receipt if stored and handled properly.