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描述 概述 The First Strand cDNA Synthesis Kit is designed for the preparation of high quality first strand cDNA from cellular RNA templates. The kit contains MMLV Reverse Transcriptase for superior yields of full-length cDNA. Both oligo(dT) and random hexamer primers are included for a choice of general priming strategies and as alternatives to user-supplied specific primers. A small volume (1-2 µl) of the first strand cDNA reaction can be used in PCR amplification with KOD Hot Start or KOD XL DNA Polymerase. Use this kit in conjunction with the Straight A’s™ mRNA Isolation System and appropriate PCR reagents to amplify rare coding regions.
产品目录编号 69001 品牌系列 Novagen®
应用数据
Figure 1. The positive control RNA was subjected to first strand cDNA synthesis with various primer combinations followed by addition of the 5'-sense Control Primer (and antisense Control Primer, where appropriate) and amplification. First strand primers are indicated. AS: antisense Control Primer, RH: random hexamers, dT: oligo (dT).产品信息 部件 安全信息 R相 R: 22-36/38
Harmful if swallowed.
Irritating to eyes and skin.S相 S: 26-36
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 Multiple storage requirements
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
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