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检测方法 Luminescence 描述 概述 A sensitive chemiluminescence-based assay kit for the qualitative detection of superoxide anions and superoxide dismutase (SOD) activity in cell cultures. Utilizes a specific enhancement reagent that amplifies the chemiluminescent signal output, and that is non-cytotoxic and non-denaturing to live cells. The principle of this assay is based on the oxidation of luminol by the superoxide anion, which produces photons of light measured with a standard luminometer. Store SOD at -20°C and all other components at 4°C. 产品目录编号 574590 品牌系列 Calbiochem®
参考 参考 Irami, K., et al. 1997. Science 275, 1649.
Stohs, S.J. 1995. J. Basic Clin. Phys. Pharmacol. 6, 205.
Tanaka, M., et al. 1994. J. Neurochem. 63, 266.
Wang, J.F., et al. 1991. Biochem. J. 279, 311.
Lomax, K.J., et al., 1989. Science 245, 409.
Vilim, V., et al. 1989, Free Rad. Biol. Med. 6, 623.
Kozumbo, W., et al. 1985. Chem.-Biol. Interact. 54, 199.
Nakazawa, A., et al. 1985. Biochem. Pharmacol. 34, 481.
Allred, C., et al. 1980. Biochem. Biophys. Acta 631, 380.产品信息 检测方法 Luminescence 形式 100 Tests 格式 Round-bottom polystyrene luminometer tubes 试剂盒包含 Enhancer Solution, Luminol Solution, Superoxide Anion (SOA) Assay Medium, Superoxide Dismutase (SOD), Xanthine Assay Medium, Xanthine Oxidase, Xanthine Solution, and a user protocol. 生物信息 化验时间 1.5 h 样品类型 Cell cultures 储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 Multiple storage requirements
存储条件 Upon arrival store the Enhancer Solution, Luminol Solution, Xanthine Solution, Xanthine Oxidase, Superoxide Assay Medium, and Xanthine Assay Medium at 4°C and the Superoxide Dismutase at -20°C. 无需冷冻 No
补充信息 试剂盒包含 Enhancer Solution, Luminol Solution, Superoxide Anion (SOA) Assay Medium, Superoxide Dismutase (SOD), Xanthine Assay Medium, Xanthine Oxidase, Xanthine Solution, and a user protocol.
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