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描述 概述 A complete mammalian proteome extraction kit (C-PEK) designed to extract virtually all proteins from both mammalian tissues and culture cells in a simple two step process within a microcentrifuge tube to minimize protein loss. The extraction results in a total protein sample that is a clarified and non-viscous protein solution showing higher resolution and an increased total number of spots in 2-D gel electrophoresis. Sample size: 1x107 - 1x108 cells or 50-100 mg tissue. 产品目录编号 539779 品牌系列 Calbiochem®
同义词 C-PEK Mammalian Kit 参考 参考 Yuan, X., et al. 2002. Electrophoresis 23, 1185.
Allen, L. 2000. Functional Genomics Nature 405 819.
Herbert, B. 1999. Electrophoresis 20, 660.产品信息 形式 20 Extractions 试剂盒包含 Wash Buffer, Resuspension Buffer, Extraction Reagent, Reducing Reagent, Benzonase® Nuclease (Cat. No. 71206), Glass Beads, and a user protocol. 生物信息 样品类型 Mammalian tissues and cultured cells 产品使用声明 预定用途 ProteoExtract™ Complete Mammalian Proteome Extraction Kit is designed to extract total proteins from mamalian tissue culture cells or mammalian tissue. 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 +2°C to +8°C
存储条件 • Wash Buffer
The Wash Buffer is provided ready-to-use. The buffer should be stored at 4°C.
• Resuspension Buffer
The Resuspension Buffer is provided ready-to-use. It can be stored at 4°C up to 6 month. For prolonged storage freeze the buffer in convenient aliquots at -20°C.
Avoid repeated freezing and thawing!
• Extraction Reagent
The Extraction Reagent is supplied as dry powder and can be stored unopened at 4°C. The Extraction Reagent should be reconstituted just prior to use as described below. After reconstitution, it can be stored at 4°C up to 6 month. For prolonged storage freeze the buffers in convenient aliquots at -20°C.
Avoid repeated freezing and thawing!
• Reducing Agent
The Reducing Agent is supplied as dry powder. It can be stored unopened at 4°C. The Reducing Agent should be reconstituted just prior to use as described below. After reconstitution, aliquot the unused material in 60 µl volumes and freeze at -20°C for further use.
Avoid repeated freezing and thawing!
• Benzonase®
Benzonase® should be stored at 4°C.避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 Wash Buffer, Resuspension Buffer, Extraction Reagent, Reducing Reagent, Benzonase® Nuclease (Cat. No. 71206), Glass Beads, and a user protocol.
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