merck millipore,默克密理博,539736,PTP1B Assay Kit, Colorimetric

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merck millipore,默克密理博,539736,PTP1B Assay Kit, Colorimetric
产品名称：PTP1B Assay Kit, Colorimetric
产品型号：539736

merck millipore,默克密理博,539736,PTP1B Assay Kit, Colorimetric

产品介绍

merck millipore,默克密理博,539736,PTP1B Assay Kit, Colorimetric

重要规格表

检测方法
Colorimetric

描述
概述	A colorimetric assay kit to measure PTP1B activity. This convenient 96-well assay is useful for screening inhibitors and modulators of PTP1B activity.
产品目录编号	539736
品牌系列	Calbiochem®
同义词	Protein Tyrosine Phosphatase 1B Assay Kit, Colorimetric

参考
参考	Elchebly, M., et al. 1999. Science 283, 1544. Puius, Y.A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 13420. Liu, F., et al. 1996. J. Biol. Chem. 271, 31290. Fujii, S., et al. 1995. Neurosci. Lett. 187, 133. Harder, K.W., et al. 1994. Biochem. J. 298, 395. Zhang, Z. Y., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 4446. Martin, B., et al. 1985. J. Biol. Chem. 260, 14932.

参考

Elchebly, M., et al. 1999. Science 283, 1544.
Puius, Y.A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 13420.
Liu, F., et al. 1996. J. Biol. Chem. 271, 31290.
Fujii, S., et al. 1995. Neurosci. Lett. 187, 133.
Harder, K.W., et al. 1994. Biochem. J. 298, 395.
Zhang, Z. Y., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 4446.
Martin, B., et al. 1985. J. Biol. Chem. 260, 14932.

产品信息
检测方法	Colorimetric
形式	96 Tests
格式	96-well plate
试剂盒包含	Human Recombinant PTP1B (Cat. No. 539735), PTP1B Substrate (Cat. No. 539737), Assay Buffer, Phosphate Detection Agent, Phosphate Standard, RK-682 Inhibitor (Cat. No. 557322), ½ Volume Plate, and a user protocol.
正控制	Recombinant PTP1B

生物信息
化验时间	2 h
样品类型	Purified enzyme

安全信息
R相	R: 36/38-21/22 Irritating to eyes and skin. Harmful in contact with skin and if swallowed.
S相	S: 26-24/25 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Avoid contact with skin and eyes.

产品使用声明
预定用途	PLEASE READ THE ENTIRE PROTOCOL BEFORE PROCEEDING WITH THE ASSAY. CAREFULLY NOTE THE HANDLING AND STORAGE CONDITIONS OF EACH KIT COMPONENT. PLEASE CONTACT CALBIOCHEM^® TECHNICAL SERVICES FOR ASSISTANCE IF NECESSARY. NOTE: THE FOLLOWING PROCEDURES ARE INTENDED ONLY AS A GUIDELINE. THE OPTIMAL EXPERIMENTAL CONDITIONS WILL VARY DEPENDING ON THE PARAMETERS BEING INVESTIGATED, AND MUST BE DETERMINED BY THE INDIVIDUAL USER. The Calbiochem^® PTP1B Assay Kit, Colorimetric is a nonradioactive assay designed for measuring PTP1B activity in purified prperations and for inhibitor or activator screening.

产品使用声明

预定用途

PLEASE READ THE ENTIRE PROTOCOL BEFORE PROCEEDING WITH THE ASSAY. CAREFULLY NOTE THE HANDLING AND STORAGE CONDITIONS OF EACH KIT COMPONENT. PLEASE CONTACT CALBIOCHEM^® TECHNICAL SERVICES FOR ASSISTANCE IF NECESSARY.

NOTE: THE FOLLOWING PROCEDURES ARE INTENDED ONLY AS A GUIDELINE. THE OPTIMAL EXPERIMENTAL CONDITIONS WILL VARY DEPENDING ON THE PARAMETERS BEING INVESTIGATED, AND MUST BE DETERMINED BY THE INDIVIDUAL USER.

The Calbiochem^® PTP1B Assay Kit, Colorimetric is a nonradioactive assay designed for measuring PTP1B activity in purified prperations and for inhibitor or activator screening.

储存和货运信息
货运代码	Dry Ice Only
毒性	Multiple Toxicity Values, refer to MSDS
储存	≤ -70°C
存储条件	Upon arrival store the 1/2 Volume Plate at room temperature and the remaining components at -70°C to maintain the highest stability. The PTP1B must be handled very carefully in order to retain maximal enzymatic activity. Thaw it quickly in a RT water bath or by rubbing between fingers, then immediately store on an ice bath. The remaining unused enzyme should be quickly refrozen by placing at -70°C. To minimize the number of freeze/thaw cycles, aliquot the PTP1B into separate tubes and store at -70°C.
避免冻结 / 解冻	Avoid freeze/thaw
无需冷冻	No

补充信息
试剂盒包含	Human Recombinant PTP1B (Cat. No. 539735), PTP1B Substrate (Cat. No. 539737), Assay Buffer, Phosphate Detection Agent, Phosphate Standard, RK-682 Inhibitor (Cat. No. 557322), ½ Volume Plate, and a user protocol.