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merck millipore,默克密理博,527460,PhosphoDetect™ Anti-PKR (pThr⁴⁵¹) Rabbit pAb
产品名称：PhosphoDetect™ Anti-PKR (pThr⁴⁵¹) Rabbit pAb
产品型号：527460
This PhosphoDetect™ Anti-PKR (pThr⁴⁵¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of PKR (pThr⁴⁵¹).
merck millipore,默克密理博,527460,PhosphoDetect™ Anti-PKR (pThr⁴⁵¹) Rabbit pAb
- 产品介绍
重要规格表
宿主 Rb 描述 概述 Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr451. Note: 1 T = 1 test. Sufficient for 10 Western miniblots. 产品目录编号 527460 品牌系列 Calbiochem®
同义词 Anti-Double-stranded RNA-dependent Protein Kinase, Phospho-Specific (Thr⁴⁵¹) 应用数据 
Detection of human PKR, phosphorylated on Thr 451, by immunoblotting. Samples: Whole cell lysates from HeLa cells left unstimulated (lane 1) or stimulated with IFNγ and calyculin (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-PKR (pThr 451) Rabbit pAb (Cat. No. 527460) (1:000) pre-incubated without peptide (lanes 1-2), non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.参考 参考 Cuddihy, A.R., et al.1999. Oncogene 8, 2690.
Srivastava, S.P., et al. 1998. J. Biol. Chem. 273, 2416.
Clemens, M.J. 1997. Int. J. Biochem. Cell Biol. 29, 945.产品信息 形式 Liquid 配方 In Dulbecco's PBS (without Mg+2 or Ca+2), 0.1% BSA, 50% glycerol, pH 7.3. 正控制 IFNγ-stimulated HeLa cells. 防腐剂 ≤0.1% sodium azide 应用 主要应用 Immunoblotting (Western Blotting)
Immunohistochemistry
生物信息 免疫原 a synthetic phosphopeptide corresponding to amino acids surrounding the Thr⁴⁵¹ phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase) 宿主 Rabbit 同种型 IgG 品种 human, mouse 储存和货运信息 货运代码 Dry Ice Only
毒性 Standard Handling
储存 ≤ -70°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-70°C).
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