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品种反应性 主要应用 宿主 格式 抗体类型 Eq, H, M, R, Ca, B WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 09-230 描述 Anti-phospho-Raptor (Thr706) Antibody Alternate Names - regulatory associated protein of mTOR
- P150 target of rapamycin (TOR)-scaffold protein
- p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats
背景信息 Raptor is a subunit of mTORC1 which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Within mTORC1, Raptor positively regulates mTORC1 activity and functions as a scaffold for recruiting mTORC1 substrates. 产品信息 格式 Affinity Purified 控制 - Modified and non modified peptides of phosphorylated and non-phosphorlyated Raptor protein
演示 Purified rabbit serum in PBS with 0.05% sodium azide. 应用 应用 Detect phospho-Raptor (Thr706) using this Anti-phospho-Raptor (Thr706) Antibody validated for use in WB, DB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human Raptor phosphorylated at and around Thr706. 表位 Ser706 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes Raptor phosphorylated at Thr706. 品种反应性 HorseHumanMouseRatCanineBovine Species Reactivity Note Reacts with human. Predicted to react with mouse, rat, dog, cow, and horse based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_065812
Entrez基因汇总 This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. 基因符号 - Raptor
- raptor
修改 - Phosphorylation
纯化方法 Affinity Purfied UniProt编号 - Q8N122
UniProt汇总 FUNCTION: Participates in the FRAP1 pathway and associates in a near stoichiometric ratio with FRAP1 to form a nutrient-sensitive complex (NSC). Plays a pivotal role as a scaffold protein in the FRAP1-signaling pathway and this interaction is essential for the catalyzed phosphorylation of EIF4EBP1. Has a positive role in nutrient-stimulated signaling to the downstream effector RPS6KB1. Under nutrient-deprived conditions, serves as a negative regulator of FRAP1 kinase activity. Regulation of the interaction with FRAP1 is a critical mechanism by which cells coordinate the rate of cell growth and maintenance of cell size with different environmental conditions.
SIZE: 1335 amino acids; 149038 Da
SUBUNIT: Binds directly 4EBP1 and RPS6KB1 independently of its association with FRAP1. Binds preferentially to poorly or non- phosphorylated form of EIF4EBP1, and this binding is critical to the ability of FRAP1 to catalyze phosphorylation. Complex with FRAP1 physically interacts under both leucine-rich and -poor conditions and therefore in at least two nutrient-determined states with different stability.
TISSUE SPECIFICITY: Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.
SIMILARITY: Contains 7 WD repeats.
MISCELLANEOUS: Rapamycin destabilizes the interaction with FRAP1 regardless of nutrient availability, and its potency for dissociation is increased under nutrient-rich conditions. This action uncouples FRAP1 from its substrates, and inhibits FRAP1 signaling without altering its intrinsic catalytic activity.产品使用声明 质量保证 Evaluated by Dot Blot in modified and non modified peptides of phosphorylated and non-phosphorlyated Raptor protein.
Dot Blot Analysis: 1 µg/mL of this antibody detected Raptor in modified and non-modified peptides of phosphorylated and non-phosphorlyated Raptor protein.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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