merck millipore,默克密理博,488250,Non-Interfering Protein Assay™ Kit

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merck millipore,默克密理博,488250,Non-Interfering Protein Assay™ Kit
产品名称：Non-Interfering Protein Assay™ Kit
产品型号：488250
Accurate protein quantification from solutions containing interfering compounds

merck millipore,默克密理博,488250,Non-Interfering Protein Assay™ Kit

产品介绍

merck millipore,默克密理博,488250,Non-Interfering Protein Assay™ Kit

描述
概述	An easy-to-use protein assay that overcomes interference of agents found in protein solutions including detergents, chelating agents, reducing agents, amines, sugars, urea, and others. The Universal Protein Precipitating Agent (UPPA™ Reagent) is used to precipitate and immobilize the protein in the tube while the interfering reagents are removed. Protein concentration is based on the specific binding of copper to the peptide backbone. As the protein concentration increases, the concentration of unbound copper ions decreases, and the color density is inversely related to the amount of protein present in solution.
产品目录编号	488250
品牌系列	Calbiochem®
应用数据

参考
参考	Ho, T.H. et al. 2005. Human Molecular Genetics 14, 1539. Ladd, A.N. et al. 2001. Mol. Cell. Biol. 21, 1285. Loeb, D.M. et al. 2002. J. Biol. Chem. 277, 19627. Shiels, A. et al. 2007. Invest. Ophthalmol. Vis. Sci. 48, 500. Gushwa, N.N. et al. 2003. Plant Physiology 132, 1925. DePinto, W. et al. 2006. Mol. Cancer Ther. 5, 2644 Werner, M.E. et al. 2007. J. Biol. Chem. 282, 5560. Dennison, S.M. et al (2006). Biophysical Journal 90, 1661. Reeve, I. et al. 2002. PNAS 99, 8608.

参考

Ho, T.H. et al. 2005. Human Molecular Genetics 14, 1539.
Ladd, A.N. et al. 2001. Mol. Cell. Biol. 21, 1285.
Loeb, D.M. et al. 2002. J. Biol. Chem. 277, 19627.
Shiels, A. et al. 2007. Invest. Ophthalmol. Vis. Sci. 48, 500.
Gushwa, N.N. et al. 2003. Plant Physiology 132, 1925.
DePinto, W. et al. 2006. Mol. Cancer Ther. 5, 2644
Werner, M.E. et al. 2007. J. Biol. Chem. 282, 5560.
Dennison, S.M. et al (2006). Biophysical Journal 90, 1661.
Reeve, I. et al. 2002. PNAS 99, 8608.

产品信息
检测方法	Colorimetric
形式	500 Tests
格式	Cuvette or 2 ml, 96-deep-well plate
试剂盒包含	UPPA™ Reagents I and II, Copper Solution I, Color Agents A and B, BSA Standard, and a user protocol.

安全信息
R相	R: 36/37/38 Irritating to eyes, respiratory system and skin.
S相	S: 26-36 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.

产品使用声明
预定用途	The Non-Interfering Protein Assay™ is a highly sensitive colorimetric assay that overcomes interference by common laboratory agents. The assay removes detergents (non-ionic, ionic and zwitterionic), reducing agents (β-mercaptoethanol, DTT), chelating agents (EDTA), amines (Tris), sugars, and is highly tolerant of strong chaotropic buffers. It is suitable for determining protein concentrations in protein loading buffer (Laemmeli buffer), high β-mercaptoethanol concentrations (<15%), and in lipid and vesicle preparations. The Non-Interfering Protein Assay™ is linear between 0.5-50 µg and requires a small sample (1-50 µl).

产品使用声明

预定用途

The Non-Interfering Protein Assay™ is a highly sensitive colorimetric assay that overcomes interference by common laboratory agents. The assay removes detergents (non-ionic, ionic and zwitterionic), reducing agents (β-mercaptoethanol, DTT), chelating agents (EDTA), amines (Tris), sugars, and is highly tolerant of strong chaotropic buffers. It is suitable for determining protein concentrations in protein loading buffer (Laemmeli buffer), high β-mercaptoethanol concentrations (<15%), and in lipid and vesicle preparations. The Non-Interfering Protein Assay™ is linear between 0.5-50 µg and requires a small sample (1-50 µl).

储存和货运信息
货运代码	Blue Ice Only
毒性	Multiple Toxicity Values, refer to MSDS
储存	+15°C to +30°C
存储条件	Upon arrival, store UPPA-I and UPPA-II at room temperature and the remaining components at 4°C, in the dark in the original box.
无需冷冻	No