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merck millipore,默克密理博,48-614MAG,MILLIPLEX MAP TGFβ Signaling Pathway Magnetic Bead 6-Plex - Cell Signaling Multiplex Assay
产品名称：MILLIPLEX MAP TGFβ Signaling Pathway Magnetic Bead 6-Plex - Cell Signaling Multiplex Assay
产品型号：48-614MAG
The analytes available for this multiplex kit are: Akt (Ser473), ERK (Thr185/Tyr187), Smad2 (Ser465/Ser467), Smad3 (Ser423/Ser425), Smad4 (Total), TGFβRII (Total).
merck millipore,默克密理博,48-614MAG,MILLIPLEX MAP TGFβ Signaling Pathway Magnetic Bead 6-Plex - Cell Signaling Multiplex Assay
- 产品介绍
- merck millipore,默克密理博,48-614MAG,MILLIPLEX MAP TGFβ Signaling Pathway Magnetic Bead 6-Plex - Cell Signaling Multiplex Assay
重要规格表
可用的分析物 品种反应性 主要应用 检测方法 TGFβRII (Total), Akt (Ser473), ERK (Thr185/Tyr187), Smad2 (Ser465/Ser467), Smad3 (Ser423/Ser425), Smad4 (Total) H Mplex Luminex xMAP 描述 产品目录编号 48-614MAG 商名 - MILLIPLEX
描述 MILLIPLEX MAP TGFβ Signaling Pathway Magnetic Bead 6-Plex - Cell Signaling Multiplex Assay 概述 Cells respond to their environment in many different ways through intracellular signaling. The TGFβ pathway plays a central role in a number of normal cellular processes including proliferation, differentiation, apoptosis, migration, adhesion, immune response and other functions in most cells. Although the TGFβ pathway regulates a wide range of processes, the pathway is fairly simple. TGFβ dimers bind to TGFβ Type II Receptor which recruits and phosphorylates TGFβ Type I Receptor. The Type I Receptor then recruits and phosphorylates SMAD2/3, which then binds to SMAD4 and forms a complex that enters the cell nucleus where it acts as a transcription factor for various genes. TGFβ Receptor activates the SMAD-dependent canonical pathway, as well as SMAD-independent non-canonical pathways, such as PI3K/Akt, ERK, JNK, and p38.
Aberrant TGFβ signaling is involved in the development of multiple diseases, including hematological malignancies such as leukemia, and impaired wound healing, neurodegenerative diseases, developmental disorders and pulmonary hypertension. Loss-of-function mutations promote tumorigenesis by suppressing the immune system and the epithelial mesenchymal transition (EMT), although TGFβ signaling has also been implicated in tumor inhibition. Immune cells such as B cells, T cells, dendritic cells and macrophages secrete TGFβ, which downregulates their proliferation, differentiation and activation by other cytokines. Consequently, modifications in TGFβ signaling have been linked to autoimmune and inflammatory diseases and cancer.产品信息 检测方法 Luminex xMAP 配置 Premixed 精度（%） - Inter-Assay: < 12
- Intra-Assay: < 8
板面类型 Cell Signaling 应用 应用 The analytes available for this multiplex kit are: Akt (Ser473), ERK (Thr185/Tyr187), Smad2 (Ser465/Ser467), Smad3 (Ser423/Ser425), Smad4 (Total), TGFβRII (Total). 主要应用 - Multiplexing
应用说明 • An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 1 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).生物信息 特异性 Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. 品种反应性 Human 可用的分析物 - TGFβRII (Total)
- Akt (Ser473)
- ERK (Thr185/Tyr187)
- Smad2 (Ser465/Ser467)
- Smad3 (Ser423/Ser425)
- Smad4 (Total)
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Recommended storage for kit components is 2 - 8°C. 包装信息 数量 96-well plate 包装 96-well plate
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