merck millipore,默克密理博,HTS115RTA,Ready-to-Assay™ M2 Acetylcholine (Muscarinic) Family Receptor Frozen Cells

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merck millipore,默克密理博,HTS115RTA,Ready-to-Assay™ M2 Acetylcholine (Muscarinic) Family Receptor Frozen Cells
产品名称：Ready-to-Assay™ M2 Acetylcholine (Muscarinic) Family Receptor Frozen Cells
产品型号：HTS115RTA
Human M2 GPCR frozen cells for Calcium Flux FLIPR Assays.

merck millipore,默克密理博,HTS115RTA,Ready-to-Assay™ M2 Acetylcholine (Muscarinic) Family Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS115RTA,Ready-to-Assay™ M2 Acetylcholine (Muscarinic) Family Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays	Human	Chem-1	M2

描述
产品目录编号	HTS115RTA
商名	Ready-to-Assay
描述	Ready-to-Assay™ M₂ Acetylcholine (Muscarinic) Family Receptor Frozen Cells
背景信息	Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. The muscarinic acetylcholine receptor (mAChR) family consists of five GPCRs that mediate some of the neurotransmission functions of acetylcholine in the CNS and the periphery. The M1, M3 and M5 receptors couple to Gq to mobilize intracellular calcium, whereas the M2 and M4 receptors couple to Gi/o to inhibit cAMP production (Caulfield and Birdsall, 1998). In urinary bladder trachea and stomach, M2 augments the function of M3 in promoting contractility, and activation of M2 serves to counteract relaxation induced by increased cAMP levels (Ehlert et al., 2005; Wess, 2004). In addition, the ability of mChR agonists to decrease heart rate appears to be mediated primarily by M2. Agonists of mAChRs induce tremor, hypothermia, corticosterone release, and analgesia; each of these functions is mediated at least in part by M2 (Wess, 2004). Millipore’s cloned human M2 -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant M2 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at M2.

产品信息
部件	Fifty (50) mL of Media Component. Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
检测方法	Fluorescent

应用
应用	Human M2 GPCR frozen cells for Calcium Flux FLIPR Assays.
应用说明	Calcium Flux Assays

生物信息
品种	Human
Entrez基因编号	NM_000739
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	M2
靶子系列	Acetylcholine (muscarinic)
细胞系类型	Ready-to-Assay

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.