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merck millipore,默克密理博,05-157,Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9
产品名称：Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9
产品型号：05-157
Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9 is an antibody against MAP Kinase 2/Erk2 for use in IP & WB.
merck millipore,默克密理博,05-157,Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, Av, H, M WB M Purified Monoclonal Antibody 描述 产品目录编号 05-157 Replaces 04-349 品牌系列 Upstate
商名 - Upstate
描述 Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9 Alternate Names - mitogen-activated protein kinase 1
- protein tyrosine kinase ERK2.
- Extracellular signal-regulated kinase 2
- MAP kinase 2
- MAPK 2
- Mitogen-activated protein kinase 2
- extracellular signal-regulated kinase-2
背景信息 ERK2 is activated by MEK2, which phosphorylates neighboring threonine 183 and tyrosine 185 residues. ERK2 is a serine/threonine kinase that phosphorylates MAP2 and myelin basic protein. This kinase is an important proximal component of the MAP kinase pathway involved in transeitting the signals from growth factors, neurotransmitters and hormones at the cell surface to the transcriptional events in the nucleus. 产品信息 格式 Purified 控制 - A431, HeLa and NIH/3T3 cell lysates
Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
演示 Purified mouse monoclonal IgG2a in buffer containing PBS with 0.05% sodium azide. Store frozen at -20°C, avoid freeze thaw cycles. 应用 应用 Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9 is an antibody against MAP Kinase 2/Erk2 for use in IP & WB. 主要应用 - Western Blotting
应用说明 Immunoprecipitation:
4 μg of a previous lot immunoprecipitated MAP Kinase 2/Erk2 from a RIPA lysate from mouse 3T3 cells. Boil the cell lysate and let cool before adding the antibody, as this antibody only binds to denatured MAP Kinase.生物信息 免疫原品种 HPLC purified murine recombinant MAP Kinase. Clone 1B3B9 克隆 1B3B9 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes MAP kinase encoded by the mapk gene, Mr 42 kDa. 同种型 IgG2a 品种反应性 RatAvianHumanMouse Species Reactivity Note Proven to react with Human, Mouse, and Rat.
Other species cross reactivity is unknown.抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002745
Entrez基因汇总 The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. 基因符号 - ERT1
- MAPK1
- p38
- ERK
- P42MAPK
- ERK2
- p40
- PRKM1
- p41mapk
- PRKM2
- MAPK2
- p42-MAPK
- p41
- ERK-2
纯化方法 Protein G chromatography UniProt编号 - P28482
UniProt汇总 FUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
COFACTOR: Magnesium (By similarity).
SIZE: 360 amino acids; 41390 Da
SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain产品使用声明 质量保证 Routinely evaluated in RIPA lysates from human A431 carcinoma cells, mouse 3T3 fibroblasts, or rat L6 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected MAP Kinase 2/Erk2 in RIPA lysates from human A431 carcinoma cells. A previous lot detected MAP Kinase in mouse 3T3 fibroblasts and rat L6 cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 200 µg
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