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描述 概述 A convenient kit for the isolation of native integral membrane and membrane-associated proteins, without the need for ultracentrifugation. Extraction is based on association of proteins with cellular membranes rather than on their hydrophobicity. Resulting samples are suitable for use in functional assays, 2-D gel electrophoresis, and other applications. Note: 1 kit is sufficient for up to 20 sample extractions. 产品目录编号 444810 品牌系列 Calbiochem®
同义词 M-PEK Kit 产品信息 形式 20 Extractions 试剂盒包含 Wash Buffer, Extraction Buffer 1, Extraction Buffer 2, Protease Inhibitor Cocktail, and a user protocol. 生物信息 样品类型 Mammalian tissues and cultured cells 产品使用声明 预定用途 The ProteoExtract™ Native Membrane Protein Extraction Kit is designed for the isolation of native membrane proteins from a broad range of mammalian samples, including:
• Adherent tissue culture cells
• Suspension grown tissue culture cells
• Frozen cell pellets
• Tissues储存和货运信息 货运代码 Shipped at Ambient Temperature or with Blue Ice
毒性 Multiple Toxicity Values, refer to MSDS
储存 +2°C to +8°C
存储条件 • Wash Buffer
Store at 4°C.
• Extraction Buffers I & II
The Extraction Buffers I and II can be stored at 4°C for up to 6 month.
For prolonged storage, freeze the buffers in convenient aliquots at -20°C. Before extraction, buffers must be thawed at room temperature (RT). After thawing, mix components by gently shaking or vortexing. Avoid repeated freezing and thawing!
• Protease Inhibitor Cocktail
The Protease Inhibitor Cocktail is supplied in DMSO and can be stored at 4°C up to 6 months. For prolonged storage, freeze the cocktail in convenient aliquots at -20°C. During the sample preparation procedure it must be kept at RT to prevent freezing of DMSO.无需冷冻 No
补充信息 试剂盒包含 Wash Buffer, Extraction Buffer 1, Extraction Buffer 2, Protease Inhibitor Cocktail, and a user protocol.
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