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描述 概述 Recombinant, human 5-Lipoxygenase expressed in S. fruigiperda insect cells using a baculovirus overexpression system. 5-LO catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4. It is localized in the cytosol of resting human and rat peripheral blood neutrophils, whereas it is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA2. 产品目录编号 437996 品牌系列 Calbiochem®
同义词 5-LO 参考 参考 Brock, T.G., et al. 1997. J. Biol. Chem. 272, 8276.
Pouliot, M., et al. 1996. Eur. J. Biochem. 238, 250.
Zhang, Y.Y., et al. 1993. J. Biol. Chem. 268, 2535.
Shimizu, T., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 689.产品信息 EC号 1.13.11.34 形式 Liquid 配方 In 100 mM Tris containing 5 mM EGTA, pH 8.0. Molar Mass 78000 生物信息 特异活性 ~20 units/mg 储存和货运信息 货运代码 Dry Ice Only
毒性 Standard Handling
储存 ≤ -70°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-70°C).
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