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检测方法 Fluorometric 描述 概述 Assay kit useful for the quantitative measurement of lecithin:cholesterol acyltransferase activity (LCAT) in human plasma. LCAT mediates the formation of cholesteryl esters in human plasma by transferring an acyl chain from the sn-2 position of phosphatidylcholine to cholesterol. 产品目录编号 428900 品牌系列 Calbiochem®
同义词 Lecithin:Cholesterol Acyltransferase Activity Assay Kit, Fluorometric 应用数据 
The non-hydrolyzed LCAT Substrate fluoresces at 470 nm. Upon hydrolysis by LCAT, a monomer is released from the LCAT Substrate that fluoresces at 390 nm. LCAT activity is assessed as a change in 470/390 emission intensity.
Normal human plasma is assayed according to the Detailed Protocol above. Over time the 470/390 ration decreases, indicating LCAT activity in the sample.产品信息 检测方法 Fluorometric 形式 100 Tests 试剂盒包含 LCAT Substrate, READ Reagent, and a user protocol. 生物信息 化验时间 4.5 - 8.5 h 样品类型 Plasma 品种 human 安全信息 R相 R: 36/38
Irritating to eyes and skin.S相 S: 26
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.产品使用声明 预定用途 The Calbiochem® LCAT Activity Assay Kit, Fluorometric is intended for the quantitative measurement of lecithin:cholesterol acyltransferase activity (LCAT) in normal human plasma. This kit allows the quantitation of fold of increase or decrease of enzymatic activity among samples, this kit also allows the quantitation of the % change of 470/390 ratio with time, which can be used to define LCAT enzymatic activity. This kit does not allow the measurement of substrate concentration or the amount of hydrolyzed substrate. 储存和货运信息 货运代码 Blue Ice Only
毒性 Irritant
储存 Multiple storage requirements
存储条件 Upon arrival store the LCAT Substrate at -20°C and the READ Reagent at 4°C. 避光 Protect from light
无需冷冻 No
补充信息 试剂盒包含 LCAT Substrate, READ Reagent, and a user protocol.
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