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描述 产品目录编号 42600 商名 - Ultrafree
描述 Ultrafree-DA 离心超滤管 概述 Recover 100 to 10,000 bp DNA from agarose gel slices in a single 10-minute spin. The kit consists of a pre-assembled filter device with an agarose gel nebulizer, a microcentrifuge vial, and modified TAE gel extraction buffer (dry).
The device utilizes gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.
Prepared DNA requires no further purification for most applications, including cloning and radioisotopic or fluorescent DNA sequencing. Since agarose gel electrophoresis has high resolving power, the small and large non-specific amplification products that frequently interfere with cloning and sequencing after PCR are completely removed from the product.- Minimal hands-on time
- 10-minute spin
- Fully-functional DNA
PerformanceTypical DNA Recoveries from Agarose Gels
DNA Size (bp) Percent DNA Recoveries
100 78
700 71
1000 77
2027 47
4361 35
9416 32
23130 29
Protocol
1. Excise the band of interest after routine electrophoretic analysis of PCR products or other DNA through an agarose gel.
2. Place the gel slice into the pre-assembled device. Note: Protocol is not compatible with low melting point agarose.
3. Spin at 5,000 x g for 10 minutes to extract gel slurry into filter cup.
4. DNA can be stored in the capped filtrate vial after discarding the sample filter cup and gel nebulizer.产品信息 色码 蓝色 最高工作温度（°C） 50 过滤器类型 微孔滤膜 应用 应用 Centrifugal Filter Unit for DNA Gel Extraction. 应用说明 固定角度 Microfuge 转子，最大离心力为 5,000 x g 生物信息 无菌性 非无菌 理化信息 离心应用 DNA Gel Extraction 尺寸 直径（mm） 10.6 过滤面积（cm²） 0.2 长度（mm） 45 体积（mL） 0.5 材料信息 装置材料 - Polypropylene
包装信息 数量 50
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