merck millipore,默克密理博,407707,PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb

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merck millipore,默克密理博,407707,PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb
产品名称：PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb
产品型号：407707
This PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb is validated for use in ELISA, WB, ICC for the detection of Insulin Receptor (pTyr1162/1163).

merck millipore,默克密理博,407707,PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb

产品介绍

merck millipore,默克密理博,407707,PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb

重要规格表

宿主
Rb

描述
概述	Recognizes the ~95 kDa β subunit of the insulin receptor protein phosphorylated at Tyr¹¹⁶² and Tyr¹¹⁶³ in insulin receptor transfected cells. Cross-reacts with the insulin-like growth factor-1 receptor phosphorylated at Tyr¹¹³⁵ and Tyr¹¹³⁶.
概述	Note: 1 T = 1 test. Sufficient for 10 Western mini blots.
产品目录编号	407707
品牌系列	Calbiochem®
应用数据	Detection of insulin receptor, phosphorylated on Tyr ^1162/1163, by immunoblotting. Samples: Whole cell lysate from CHO-T cells transfected with an insulin receptor containing vector and left unstimulated (lane 1), or stimulated with insulin (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Insulin Receptor (pTyr^1162/1163) Rabbit pAb (Cat. No. 407707) (1:1000) pre-incubated without peptide (lanes 1, 5), with phosphopeptide immunogen (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide (lane 3), or a generic phosphotyrosine-containing peptide (lane 4). Detection: chemiluminescence.

参考
参考	Bevan, P. 2001. J. Cell Sci. 114, 1429. Ottensmeyer, F.P., et al. 2000. Biochemistry 39, 12103. Virkamaki, A., et al. 1999. J. Clin. Invest. 103, 931. Schmid, E., at al. 1998. FASEB J. 12, 863. Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341. Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 328. White, M.F. and Kahn, C.R. 1986. The Enzymes 17, 247. Ebina, Y., et al. 1985. Cell 40, 747. Ganguly, S., et al. 1985. Current Topics in Cellular Regulation 27, 83. Grunberger, G., et al. 1984. Science 223, 932.

参考

Bevan, P. 2001. J. Cell Sci. 114, 1429.
Ottensmeyer, F.P., et al. 2000. Biochemistry 39, 12103.
Virkamaki, A., et al. 1999. J. Clin. Invest. 103, 931.
Schmid, E., at al. 1998. FASEB J. 12, 863.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 328.
White, M.F. and Kahn, C.R. 1986. The Enzymes 17, 247.
Ebina, Y., et al. 1985. Cell 40, 747.
Ganguly, S., et al. 1985. Current Topics in Cellular Regulation 27, 83.
Grunberger, G., et al. 1984. Science 223, 932.

产品信息
形式	Liquid
配方	In Dulbecco's PBS (without Mg²⁺ and Ca²⁺), 50% glycerol, 1 mg/ml BSA, pH 7.3.
防腐剂	≤0.1% sodium azide

应用
主要应用	Enzyme-Linked Immunosorbent Assay Immunoblotting (Western Blotting) Immunocytochemistry

生物信息
免疫原	a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr^1162/1163 phosphorylation sites of human insulin receptor
免疫原品种	Human
宿主	Rabbit
同种型	IgG
品种	human

储存和货运信息
货运代码	Dry Ice Only
毒性	Standard Handling
储存	≤ -70°C
避免冻结 / 解冻	Avoid freeze/thaw
无需冷冻	No
特殊指南	Following initial thaw, aliquot and freeze (-20°C).