merck millipore,默克密理博,400050,Hygromycin B, Streptomyces sp., Cell Culture-Tested - CAS 31282-04-9

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merck millipore,默克密理博,400050,Hygromycin B, Streptomyces sp., Cell Culture-Tested - CAS 31282-04-9 - Calbiochem
产品名称：Hygromycin B, Streptomyces sp., Cell Culture-Tested - CAS 31282-04-9 - Calbiochem
产品型号：400050
Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells.

merck millipore,默克密理博,400050,Hygromycin B, Streptomyces sp., Cell Culture-Tested - CAS 31282-04-9 - Calbiochem

产品介绍

merck millipore,默克密理博,400050,Hygromycin B, Streptomyces sp., Cell Culture-Tested - CAS 31282-04-9 - Calbiochem

重要规格表

经验配方	CAS号
C₂₀H₃₇N₃O₁₃	31282-04-9

描述
概述	Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for the identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent. Working concentration: 50 µg/ml - 1 mg/ml for mammalian cell selection.
概述	Potency: ≥1000 µ/mg solid.
产品目录编号	400050
品牌系列	Calbiochem®

参考
参考	Dean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287. Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717. Rikkerink, E. H., et al. 1994. Current Genetics 25, 202. Sugimoto, K., et al. 1994. Plant J. 5, 863. MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67. Bilang, R., et al. 1991. Gene 100, 247. Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255. Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423. MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125. Zhou, J., et al. 1991. Gene 107, 307. Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155. Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285. Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915. Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311. Crespi, C.L., et al. 1989. Carcinogenesis 10, 295. Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6. Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965. Kronstad, J.W., et al. 1989. Gene 79, 97. Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118. Cullen, D., et al. 1987. Gene 57, 21. Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459. Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6. Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.

参考

Dean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
Sugimoto, K., et al. 1994. Plant J. 5, 863.
MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
Bilang, R., et al. 1991. Gene 100, 247.
Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
Zhou, J., et al. 1991. Gene 107, 307.
Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
Kronstad, J.W., et al. 1989. Gene 79, 97.
Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
Cullen, D., et al. 1987. Gene 57, 21.
Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.

产品信息
CAS号	31282-04-9
形式	Light brown lyophilized solid
Hill配方	C₂₀H₃₇N₃O₁₃
化学配方	C₂₀H₃₇N₃O₁₃
Molar Mass	527.5
结构配方图像

生物信息
生物活性	The activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard.
纯度	≥90% by HPLC and TLC

理化信息
溶解度	dH₂O

GHS安全信息
RTECS	WK2130000

安全信息
R相	R: 26/27/28-37/38-41 Very toxic by inhalation, in contact with skin and if swallowed. Irritating to respiratory system and skin. Risk of serious damage to eyes.
S相	S: 22-26-36/37/39-45 Do not breathe dust. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).

储存和货运信息
货运代码	Ambient Temperature Only
毒性	Highly Toxic & Carcinogenic / Teratogenic
有害材料注意事项：	Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存	+2°C to +8°C
无需冷冻	No
特殊指南	Aqueous stock solutions should be kept at concentrations of ≥50 mg/ml and can be stored at either 4°C or -20°C. Aqueous stock solutions (≥50 mg/ml) are stable for up to 6 months at 4°C or -20°C. Working solutions (< 2 mg/ml) are stable for up to 1 month at 4°C. Note: This product is highly toxic. Read enclosed MSDS before handling.