merck millipore,默克密理博,17-141,TUNEL Apoptosis Detection Kit

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Merck Millipore 默克密理愽

主要应用	检测方法
IF	Fluorescent

描述
产品目录编号	17-141
品牌系列	Upstate
商名	Upstate
描述	TUNEL Apoptosis Detection Kit
概述	For the detection of the endonucleolytic cleavage of chromatin, characteristic of apoptosis.
背景信息	Most animal cells can self-destruct via an intrinsic program of cell death that may be triggered by extracellular or intracellular signals. Programmed cell death plays an important role in many biological processes such as morphogenesis and negative selection in the immune system. In addition, dysregulation of programmed cell death may contribute to diseases, for example, cancer, neurodegenerative diseases, and AIDS. Apoptosis is the term for programmed cell death characterized by specific morphologic and biochemical properties. Apoptotic cells typically undergo a series of structural changes: blebbing of the plasma membrane; condensation of the cytoplasm and nuclei; and cellular fragmentation into apoptotic bodies, membrane-bound structures containing condensed cytoplasm, some intact organelles, and nuclear fragments. The most common biochemical property of apoptosis is the endonucleolytic cleavage of chromatin, initially to large fragments of 50-300 kilobase pairs and subsequently to monomers and multimers of 180-200 base pairs.

产品信息
部件	Bovine DNase I, Catalog # 17-141h. One vial containing 200 μg in 100 μL. Proteinase K (PK), Catalog # 17-141a. One vial containing 400 μg in 400 μL. TdT (Terminal deoxynucleotidyl Transferase), Catalog # 17-141b. One vial containing 300 Units in 100 μL. TdT Buffer, Catalog # 17-141c. Three vials, each vial containing 2 mL. Biotin-dUTP (biotinylated deoxyuridine riphosphate), Catalog # 17-141d. One vial containing 100 μL of 250 μM solution. TB Buffer (10X), (termination buffer), Catalog # 17-141e. One bottle containing 50 mL of 10X solution. Blocking Solution, Catalog # 17-141f. One vial containing 2 mL. Avidin-FITC, Catalog # 17-141g. One vial containing 10 μg in 200 μL.
检测方法	Fluorescent

产品信息

部件

Bovine DNase I, Catalog # 17-141h. One vial containing 200 μg in 100 μL.
Proteinase K (PK), Catalog # 17-141a. One vial containing 400 μg in 400 μL.
TdT (Terminal deoxynucleotidyl Transferase), Catalog # 17-141b. One vial containing 300 Units in 100 μL.
TdT Buffer, Catalog # 17-141c. Three vials, each vial containing 2 mL.
Biotin-dUTP (biotinylated deoxyuridine riphosphate), Catalog # 17-141d. One vial containing 100 μL of 250 μM solution.
TB Buffer (10X), (termination buffer), Catalog # 17-141e. One bottle containing 50 mL of 10X solution.
Blocking Solution, Catalog # 17-141f. One vial containing 2 mL.
Avidin-FITC, Catalog # 17-141g. One vial containing 10 μg in 200 μL.

检测方法

Fluorescent

应用
应用	TUNEL Apoptosis Detection Kit for the detection of the endonucleolytic cleavage of chromatin, characteristic of apoptosis.
主要应用	Immunofluorescence

生物信息
品种反应性	Mammals

产品使用声明
质量保证	Routinely evaluated by detecting cleaved DNA in HeLa cells after incubation with bovine DNase I
销售限制	This product is not available for sale in Japan.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of shipment. Once thawed, Blocking solution (Catalog #17-141f) and TB Buffer (Catalog # 17-141e) should be stored at 4°C.