merck millipore,默克密理博,371206,Dkk-1, His•Tag, Mouse, Recombinant

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merck millipore,默克密理博,371206,Dkk-1, His•Tag, Mouse, Recombinant
产品名称：Dkk-1, His•Tag, Mouse, Recombinant
产品型号：371206

merck millipore,默克密理博,371206,Dkk-1, His•Tag, Mouse, Recombinant

产品介绍

merck millipore,默克密理博,371206,Dkk-1, His•Tag, Mouse, Recombinant

重要规格表

纯度
≥95% by SDS-PAGE

描述
概述	Recombinant, murine Dickkopf related protein-1 (Dkk-1), consisting of amino acids 30-272, fused at the C-terminus to a His•Tag^® sequence, and expressed in NS0-dervied mouse myeloma cells. Dkk-1 is a well-documented antagonist of the Wnt signaling pathway. The balance between Dkk-1 inhibition and Wnt signaling is essential for bone formation and homeostasis.
产品目录编号	371206
品牌系列	Calbiochem®

参考
参考	Pinzone, J.J. et al. 2009. Blood 113, 517. Li, J. et al. 2006. Bone 36, 754. Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934. Niehrs, C. 2006. Oncogene 25, 7469. Verani, R. et al. 2006. J. Neurochem. 101, 242. Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647. Kemp, C. et al. 2005. Dev. Dyn. 233, 1064. Adamska, M. et al. 2004. Dev. Biol. 272, 134. Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582. Mao, B. et al. 2002. Nature 417, 664. Mao, B. et al. 2001. Nature 411, 321. Glinka, A. et al. 1998. Nature 391, 357.

参考

Pinzone, J.J. et al. 2009. Blood 113, 517.
Li, J. et al. 2006. Bone 36, 754.
Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
Niehrs, C. 2006. Oncogene 25, 7469.
Verani, R. et al. 2006. J. Neurochem. 101, 242.
Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
Adamska, M. et al. 2004. Dev. Biol. 272, 134.
Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
Mao, B. et al. 2002. Nature 417, 664.
Mao, B. et al. 2001. Nature 411, 321.
Glinka, A. et al. 1998. Nature 391, 357.

产品信息
形式	Lyophilized
配方	Lyophilized from 0.2 µm filtered PBS, BSA.
Molar Mass	27600

生物信息
生物活性	ND₅₀: 0.2-1 µg/ml as measured by the inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells. Reduction of Axis-inducing activity: 60-100% reduction (using 1 ng rmDkk-1 per embryo) in the number of secondary axes produced in the presence of 10-20 pg Xwnt-8 mRNA using early Xenopus embryos.
纯度	≥95% by SDS-PAGE

理化信息
污染物	Endotoxin: ≤1.0 EU/µg protein
溶解度	PBS

储存和货运信息
货运代码	Ambient Temperature Only
毒性	Regulatory Review
储存	-20°C
无需冷冻	No
特殊指南	Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.