merck millipore,默克密理博,05-784R,Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal

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merck millipore,默克密理博,05-784R,Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal
产品名称：Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal
产品型号：05-784R
Detect IRS1 using this Anti-IRS1 Antibody, clone 58-10C-31 validated for use in WB & IP.

merck millipore,默克密理博,05-784R,Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal

产品介绍

merck millipore,默克密理博,05-784R,Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H, M, R	WB	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	05-784R
描述	Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal
Alternate Names	insulin receptor substrate 1
背景信息	IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. It is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer.

产品信息
格式	Purified
控制	3T3/A31, 3T3/L1, L6, or MCF7 cell lysates
演示	Purified Rabbit Monoclonal IgG Supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 40% glycerol.

应用
应用	Detect IRS1 using this Anti-IRS1 Antibody, clone 58-10C-31 validated for use in WB & IP.
主要应用	Western Blotting
应用说明	Western Blot (SNAP ID) Analysis: 0.1 µg/mL from a previous lot detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6 and MCF7 cell lysates. Immunoprecipitation Analysis: 1 µg from a previous lot immunoprecipitated IRS-1 from 100 µg of MCF7 cell lysate. Arrow indicates IRS-1 (~160 kDa). There is cross reactivity of the detection antibody with the heavy chaing of Rabbit IgG as shown at ~50 kDa.

生物信息
免疫原品种	KLH- conjugated - linear peptide corresponding to the C-terminus of rat IRS1.
表位	C-terminus
克隆	58-10C-31
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Antibody recognizes the C-terminus of IRS1.
同种型	IgG
品种反应性	HumanMouseRat
Species Reactivity Note	Reacts with mouse, rat and human.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_0371
基因符号	HIRS-1 IRS1 IRS-1
纯化方法	Protein A purfied
UniProt编号	P35570
UniProt汇总	FUNCTION: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. Ref.3 SUBUNIT STRUCTURE: Interacts with SOCS7 By similarity. Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-307 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor, and Ser-789 phosphorylation is increased in the liver of insulin-resistant rats. Phosphorylation of Tyr-895 is required for GRB2-binding. SEQUENCE SIMILARITIES: Contains 1 IRS-type PTB domain. Contains 1 PH domain.

产品使用声明
质量保证	Evaluated by Western Blot in 3T3/A31, 3T3/L1, L6 or MCF7 cell lysates. Western Blot Analysis: 0.1 µg/mL of this antibody detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6, or MCF7 cell lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.