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- merck millipore,默克密理博,324727,Endoglycosidase F3, Elizabethkingia meningosepticum, Recombinant, E. coli
重要规格表
描述 概述 Recombinant, Elizabethkingia meningosepticum endoglycosidase F3 expressed in E. coli. Cleaves asparagine-linked or free biantennary and triantennary complex, and Man3GlcNAc oligosaccharides from glycoproteins. This enzyme cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine residue. Core fucosylation increases the activity of Endo F3 up to 40 fold. Exhibits no activity on high mannose and hybrid molecules. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins. Note: 1 mU = 1 milliunit. 产品目录编号 324727 品牌系列 Calbiochem®
同义词 Endo-β-N-acetylglucosaminidase F3, Endo F3 参考 参考 Tarentino, A.L., et al. 1995. Glycobiology 5, 599.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.产品信息 活性 ≥5 units/ml EC号 3.2.1.96 形式 Liquid 配方 In 20 mM Tris-HCl, pH 7.5. Molar Mass 32000 生物信息 特异活性 ≥30 units/mg protein 理化信息 污染物 Proteases: none detected 储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 +2°C to +8°C
无需冷冻 Yes
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