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描述 概述 Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis. 产品目录编号 324715 品牌系列 Calbiochem®
参考 参考 Jekel, P.A., et al. 1983. Anal. Biochem. 134, 347. 产品信息 CAS号 72561-05-8 EC号 3.4.21.50 形式 Lyophilized 配方 Lyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0. Molar Mass 33000 吸湿 Hygroscopic
生物信息 纯度 ≥90% by SDS-PAGE 特异活性 ≥200 units/mg protein 理化信息 pH值 optimum 8.5 - 8.8 溶解度 H₂O 安全信息 R相 R: 20-36/37/38-42/43
Harmful by inhalation.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.S相 S: 22-26-36
Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.储存和货运信息 货运代码 Ambient Temperature Only
毒性 Harmful
储存 +2°C to +8°C
吸湿 Hygroscopic
无需冷冻 No
特殊指南 Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
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