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描述 概述 Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis. 产品目录编号 324708 品牌系列 Calbiochem®
参考 参考 Drapeau, G.R. 1980. J. Biol. Chem. 255, 839. 产品信息 CAS号 9001-92-7 EC号 3.4.24.33 形式 White lyophilized solid 配方 Lyophilized from 10 mM Tris-HCl, pH 7.5. Molar Mass 27000 生物信息 纯度 ≥90% by SDS-PAGE 特异活性 ≥20,000 units/mg protein 理化信息 溶解度 H₂O 安全信息 R相 R: 20-36/37/38-42/43
Harmful by inhalation.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.S相 S: 22-26-36
Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.储存和货运信息 货运代码 Ambient Temperature Only
毒性 Harmful
储存 +2°C to +8°C
无需冷冻 No
特殊指南 Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
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