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经验配方 CAS号 C₂₁H₂₁N₃ 38483-26-0 描述 概述 A cell-permeable, chemically-reduced ethidium derivative. Commonly used to analyze respiratory burst in phagocytes. Useful fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, whereas after oxidation of this probe to ethidium, it intercalates cell’s DNA, staining the nucleus with a bright red fluorescence. 产品目录编号 309800 品牌系列 Calbiochem®
同义词 2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, DHE 参考 参考 Haugen, T.S., et al. 1999. Eur. Respir. J. 14, 1100.
Mancini, M., et al. 1997. J. Cell Biol. 138, 449.
Carter, W.O., et al. 1994. J. Leukoc. Biol. 55, 253.
Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.
Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.
Perticarari, S., et al. 1991. Cytometry 12, 687.
Bucana, C.D., et al. 1990. Exp. Cell Res. 190, 69.
Rothe, G., and Valet, G. 1990. J. Leukoc. Biol. 47, 440.产品信息 CAS号 38483-26-0 具有ATP竞争性 N 形式 Pink solid Hill配方 C₂₁H₂₁N₃ 化学配方 C₂₁H₂₁N₃ Molar Mass 315.4 可逆 N 结构配方图像 生物信息 主靶 Useful fluorogenic probe for the detection of reactive oxygen species (ROS) 纯度 ≥95% by HPLC 理化信息 细胞可渗透 Y 溶解度 DMF or DMSO GHS安全信息 RTECS SF7935000 安全信息 R相 R: 36/37/38
Irritating to eyes, respiratory system and skin.S相 S: 26-36
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.储存和货运信息 货运代码 Ambient Temperature Only
毒性 Irritant
储存 -20°C
避光 Protect from light
无需冷冻 No
特殊指南 Following reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. 包装信息 惰性气体下包装 Packaged under inert gas
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